Abstract
Donors after cardiac death present a significant pool of untapped organs for transplantation, and use of machine perfusion strategies has been an active focus area in experimental transplantation. However, despite 2 decades of research, a gold standard has yet to emerge for machine perfusion systems and protocols. Whole blood reperfusion has been used as a surrogate for organ transplantation, especially as a model for the short-term response posttransplantation, and for optimization of perfusion systems. Although it is known that there is a strong correlation between liver function in whole-blood reperfusion and survival, the exact nature of these correlations, and to what extent they can be considered as an indicator of viability for transplantation/recipient survival, remain unclear. In this work, we demonstrate that diluted whole-blood reperfusion can be used as a direct model for transplantation of ischemic rat liver grafts. Specifically, we show that recipient survival can be predicted based simply on the value of alanine aminotransferase during perfusion, providing quantitative criteria of viability for use in this animal model. These results indicate that in the rat model graft survival is highly correlated with hepatocellular damage.
| Original language | English |
|---|---|
| Pages (from-to) | 2463-2467 |
| Number of pages | 5 |
| Journal | Transplantation Proceedings |
| Volume | 42 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2010 Sep |
| Externally published | Yes |
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ASJC Scopus subject areas
- Surgery
- Transplantation
Cite this
Diluted blood reperfusion as a model for transplantation of ischemic rat livers : Alanine aminotransferase is a direct indicator of viability. / Uygun, K.; Tolboom, H.; Izamis, M. L.; Uygun, B.; Sharma, N.; Yagi, Hiroshi; Soto-Gutierrez, A.; Hertl, M.; Berthiaume, F.; Yarmush, M. L.
In: Transplantation Proceedings, Vol. 42, No. 7, 09.2010, p. 2463-2467.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Diluted blood reperfusion as a model for transplantation of ischemic rat livers
T2 - Alanine aminotransferase is a direct indicator of viability
AU - Uygun, K.
AU - Tolboom, H.
AU - Izamis, M. L.
AU - Uygun, B.
AU - Sharma, N.
AU - Yagi, Hiroshi
AU - Soto-Gutierrez, A.
AU - Hertl, M.
AU - Berthiaume, F.
AU - Yarmush, M. L.
PY - 2010/9
Y1 - 2010/9
N2 - Donors after cardiac death present a significant pool of untapped organs for transplantation, and use of machine perfusion strategies has been an active focus area in experimental transplantation. However, despite 2 decades of research, a gold standard has yet to emerge for machine perfusion systems and protocols. Whole blood reperfusion has been used as a surrogate for organ transplantation, especially as a model for the short-term response posttransplantation, and for optimization of perfusion systems. Although it is known that there is a strong correlation between liver function in whole-blood reperfusion and survival, the exact nature of these correlations, and to what extent they can be considered as an indicator of viability for transplantation/recipient survival, remain unclear. In this work, we demonstrate that diluted whole-blood reperfusion can be used as a direct model for transplantation of ischemic rat liver grafts. Specifically, we show that recipient survival can be predicted based simply on the value of alanine aminotransferase during perfusion, providing quantitative criteria of viability for use in this animal model. These results indicate that in the rat model graft survival is highly correlated with hepatocellular damage.
AB - Donors after cardiac death present a significant pool of untapped organs for transplantation, and use of machine perfusion strategies has been an active focus area in experimental transplantation. However, despite 2 decades of research, a gold standard has yet to emerge for machine perfusion systems and protocols. Whole blood reperfusion has been used as a surrogate for organ transplantation, especially as a model for the short-term response posttransplantation, and for optimization of perfusion systems. Although it is known that there is a strong correlation between liver function in whole-blood reperfusion and survival, the exact nature of these correlations, and to what extent they can be considered as an indicator of viability for transplantation/recipient survival, remain unclear. In this work, we demonstrate that diluted whole-blood reperfusion can be used as a direct model for transplantation of ischemic rat liver grafts. Specifically, we show that recipient survival can be predicted based simply on the value of alanine aminotransferase during perfusion, providing quantitative criteria of viability for use in this animal model. These results indicate that in the rat model graft survival is highly correlated with hepatocellular damage.
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UR - http://www.scopus.com/inward/citedby.url?scp=77956457930&partnerID=8YFLogxK
U2 - 10.1016/j.transproceed.2010.04.037
DO - 10.1016/j.transproceed.2010.04.037
M3 - Article
C2 - 20832525
AN - SCOPUS:77956457930
VL - 42
SP - 2463
EP - 2467
JO - Transplantation Proceedings
JF - Transplantation Proceedings
SN - 0041-1345
IS - 7
ER -