Diquafosol sodium ophthalmic solution for the treatment of dry eye: clinical evaluation and biochemical analysis of tear composition

Chika Shigeyasu, Masakazu Yamada, Yoko Akune, Kazuo Tsubota

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Purpose: To evaluate the clinical efficacy of 3 % diquafosol sodium ophthalmic solution for dry eye, and to analyze the concentration of tear proteins and mucin-like substances after the treatment. Methods: Fifty eyes of 25 patients with dry eye syndrome were prospectively enrolled. The patients were treated with diquafosol solution at a dose of 1 drop in each eye 6 times daily for 4 weeks. The parameters of clinical efficacy were tear osmolarity, tear breakup time (BUT), fluorescein staining scores for the cornea and conjunctiva, Schirmer test values, and subjective symptoms evaluated using the ocular surface disease index (OSDI). Tears collected with Schirmer test strips were analyzed by high-performance liquid chromatography, and the concentrations of the total protein and the 4 major tear proteins, namely, secretory IgA, lactoferrin, lipocalin-1, lysozyme, and N-acetyl-neuraminic acid (Neu5Ac), were measured. Neu5Ac is a major sialic acid, a marker of secretory mucins. Results: The BUT, keratoconjunctival staining scores, and Schirmer test values were improved with statistical significance after the treatment with diquafosol solution, while changes in the other parameters, including tear osmolarity, corneal staining scores, and OSDI scores were not significant. The Neu5Ac concentration was significantly increased, which was not accompanied by changes in tear proteins. Conclusions: Topical application of diquafosol significantly improved the clinical parameters of the BUT, keratoconjunctival staining scores, and Schirmer test values and was accompanied by increased sialic acid content in the tears of patients with dry eye.

Original languageEnglish
Pages (from-to)415-420
Number of pages6
JournalJapanese Journal of Ophthalmology
Volume59
Issue number6
DOIs
Publication statusPublished - 2015 Nov 1

Fingerprint

Ophthalmic Solutions
Tears
Sodium
Staining and Labeling
Eye Diseases
N-Acetylneuraminic Acid
Mucins
Osmolar Concentration
Lipocalin 1
Neuraminic Acids
Dry Eye Syndromes
Therapeutics
Secretory Immunoglobulin A
Lactoferrin
Conjunctiva
Muramidase
Fluorescein
Cornea
High Pressure Liquid Chromatography
diquafosol

Keywords

  • Diquafosol ophthalmic solution
  • Dry eye syndrome
  • Mucins
  • Sialic acid
  • Tears

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Diquafosol sodium ophthalmic solution for the treatment of dry eye : clinical evaluation and biochemical analysis of tear composition. / Shigeyasu, Chika; Yamada, Masakazu; Akune, Yoko; Tsubota, Kazuo.

In: Japanese Journal of Ophthalmology, Vol. 59, No. 6, 01.11.2015, p. 415-420.

Research output: Contribution to journalArticle

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