Direct cell surface modification with DNA for the capture of primary cells and the investigation of myotube formation on defined patterns

Sonny C. Hsiao, Betty J. Shum, Hiroaki Onoe, Erik S. Douglas, Zev J. Gartner, Richard A. Mathies, Carolyn R. Bertozzi, Matthew B. Francis

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Previously, we reported a method for the attachment of living cells to surfaces through the hybridization of synthetic DNA strands attached to their plasma membrane. The oligonucleotides were introduced using metabolic carbohydrate engineering, which allowed reactive tailoring of the cell surface glycans for chemoselective bioconjugation. While this method is highly effective for cultured mammalian cells, we report here a significant improvement of this technique that allows the direct modification of cell surfaces with NHS-DNA conjugates. This method is rapid and efficient, allowing virtually any mammalian cell to be patterned on surfaces bearing complementary DNA in under 1 h. We demonstrate this technique using several types of cells that are generally incompatible with integrin-targeting approaches, including red blood cells and primary T-cells. Cardiac myoblasts were also captured. The immobilization procedure itself was found not to activate primary T-cells, in contrast to previously reported antibody- and lectin-based methods. Myoblast cells were patterned with high efficiency and remained undifferentiated after surface attachment. Upon changing to differentiation media, myotubes formed in the center of the patterned areas with an excellent degree of edge alignment. The availability of this new protocol greatly expands the applicability of the DNA-based attachment strategy for the generation of artificial tissues and the incorporation of living cells into device settings.

Original languageEnglish
Pages (from-to)6985-6991
Number of pages7
JournalLangmuir
Volume25
Issue number12
DOIs
Publication statusPublished - 2009 Jun 16
Externally publishedYes

Fingerprint

Surface treatment
DNA
deoxyribonucleic acid
Cells
attachment
cells
T-cells
Bearings (structural)
complementary DNA
oligonucleotides
Oligonucleotides
carbohydrates
erythrocytes
Cell membranes
Carbohydrates
antibodies
immobilization
cultured cells
Lectins
Antibodies

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Direct cell surface modification with DNA for the capture of primary cells and the investigation of myotube formation on defined patterns. / Hsiao, Sonny C.; Shum, Betty J.; Onoe, Hiroaki; Douglas, Erik S.; Gartner, Zev J.; Mathies, Richard A.; Bertozzi, Carolyn R.; Francis, Matthew B.

In: Langmuir, Vol. 25, No. 12, 16.06.2009, p. 6985-6991.

Research output: Contribution to journalArticle

Hsiao, SC, Shum, BJ, Onoe, H, Douglas, ES, Gartner, ZJ, Mathies, RA, Bertozzi, CR & Francis, MB 2009, 'Direct cell surface modification with DNA for the capture of primary cells and the investigation of myotube formation on defined patterns', Langmuir, vol. 25, no. 12, pp. 6985-6991. https://doi.org/10.1021/la900150n
Hsiao, Sonny C. ; Shum, Betty J. ; Onoe, Hiroaki ; Douglas, Erik S. ; Gartner, Zev J. ; Mathies, Richard A. ; Bertozzi, Carolyn R. ; Francis, Matthew B. / Direct cell surface modification with DNA for the capture of primary cells and the investigation of myotube formation on defined patterns. In: Langmuir. 2009 ; Vol. 25, No. 12. pp. 6985-6991.
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