Direct chiral resolution of malic acid in apple juice by ligand-exchange capillary electrophoresis using copper(II)-L-tartaric acid as a chiral selector

Shuji Kodama, Atsushi Yamamoto, Akinobu Matsunaga, Tomoyoshi Soga, Kazuichi Hayakawa

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Chiral resolution of native DL-malic acid was achieved by ligand-exchange capillary electrophoresis using copper(II)-L-tartrate as a chiral selector. Factors affecting chiral resolution, migration time, and peak area of malic acid were studied. The running conditions for optimum separation of malic acid were found to be 1 mM copper(II) sulfate-1 mM L-tartrate (pH 5.1) with an effective voltage of-20 kV at 30°C, using direct detection at 280 nm, and resolution (Rs) of racemic malic acid was approximately 4. With this system, D- and L-malic acids in apple juice were analyzed successfully.

Original languageEnglish
Pages (from-to)3286-3290
Number of pages5
JournalElectrophoresis
Volume22
Issue number15
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Malus
Copper
Ligands
Copper Sulfate
Sulfates
malic acid
tartaric acid
Electric potential

Keywords

  • Apple juice
  • Capillary electrophoresis
  • Chiral resolution
  • Ligand exchange
  • Malic acid

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Direct chiral resolution of malic acid in apple juice by ligand-exchange capillary electrophoresis using copper(II)-L-tartaric acid as a chiral selector. / Kodama, Shuji; Yamamoto, Atsushi; Matsunaga, Akinobu; Soga, Tomoyoshi; Hayakawa, Kazuichi.

In: Electrophoresis, Vol. 22, No. 15, 2001, p. 3286-3290.

Research output: Contribution to journalArticle

Kodama, Shuji ; Yamamoto, Atsushi ; Matsunaga, Akinobu ; Soga, Tomoyoshi ; Hayakawa, Kazuichi. / Direct chiral resolution of malic acid in apple juice by ligand-exchange capillary electrophoresis using copper(II)-L-tartaric acid as a chiral selector. In: Electrophoresis. 2001 ; Vol. 22, No. 15. pp. 3286-3290.
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