Direct chiral resolution of malic acid in apple juice by ligand-exchange capillary electrophoresis using copper(II)-L-tartaric acid as a chiral selector

Shuji Kodama, Atsushi Yamamoto, Akinobu Matsunaga, Tomoyoshi Soga, Kazuichi Hayakawa

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Abstract

Chiral resolution of native DL-malic acid was achieved by ligand-exchange capillary electrophoresis using copper(II)-L-tartrate as a chiral selector. Factors affecting chiral resolution, migration time, and peak area of malic acid were studied. The running conditions for optimum separation of malic acid were found to be 1 mM copper(II) sulfate-1 mM L-tartrate (pH 5.1) with an effective voltage of-20 kV at 30°C, using direct detection at 280 nm, and resolution (Rs) of racemic malic acid was approximately 4. With this system, D- and L-malic acids in apple juice were analyzed successfully.

Original languageEnglish
Pages (from-to)3286-3290
Number of pages5
JournalElectrophoresis
Volume22
Issue number15
DOIs
Publication statusPublished - 2001
Externally publishedYes

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Keywords

  • Apple juice
  • Capillary electrophoresis
  • Chiral resolution
  • Ligand exchange
  • Malic acid

ASJC Scopus subject areas

  • Clinical Biochemistry

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