Direct determination of E2020 enantiomers in plasma by liquid chromatography-mass spectrometry and column-switching techniques

Kenji Matsui, Yoshiya Oda, Hiroshi Ohe, Shigeru Tanaka, Naoki Asakawa

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

High-performance liquid chromatography with column switching and mass spectrometry (MS) was applied to the on-line determination and resolution of the enantiomers of E2020 (acetylcholinesterase inhibitor) in plasma. This system employs two avidin columns and fast atom bombardment (FAB)-MS. A plasma sample was injected directly into an avidin trapping column (10 mm × 40 mm I.D.). The plasma protein was washed out from the trapping column immediately while E2020 was retained. After the column-switching procedure, E2020 was separated enantioselectivity in an avidin analytical column. The separated E2020 enantiomers were specifically detected by FAB-MS without interference from metabolites of E2020 and plasma constituents. The limit of quantification for each enantiomer of E2020 in plasma was 1.0 ng/ml and the intra- and inter-assay relative standard deviations for the method were less than 5.2%. The assay was validated for enantioselective pharmacokinetic studies in the dog.

Original languageEnglish
Pages (from-to)209-218
Number of pages10
JournalJournal of Chromatography A
Volume694
Issue number1
DOIs
Publication statusPublished - 1995 Mar 3

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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