Direct influences of interferon-γ on proliferation and differentiation of cancer cells

T. Yano, S. Kuninaka, T. Kanematsu, T. Uehara, Y. Terazaki, Y. Fukuyama, H. Yokoyama, H. Asoh, Y. Ichinose

Research output: Contribution to journalArticle

Abstract

In order to clarify the anti-tumor activity of IFN-γ, we investigated the direct influence of IFN-γ on both the growth and cell-surface antigen expression of tumor cells. In the present study, four human lung cancer cell lines were used; two squamous cell lines (QG-56, QG-95) and two adenocarcinoma cell lines (PC-9, PC-12). The proliferation of QG-56 or QG-95 was inhibited by IFN-γ in a dose-dependent manner with about 70% inhibition at 1,000 JRU/ml while that of PC-9 was slightly inhibited with maximally 25% inhibition at 1,000 JRU/ml. The growth of PC-12 was not inhibited at all. After culture in the presence of IFN-γ (200 JRU/ml) for 14-16 days, tumor cells were examined for expression of various antigens, including HLA-class I, HLA-class II, and CEA. In all cell lines but PC-12, 100% of cells expressed HLA-class I after incubation with IFN-γ. Both HLA-class II and CEA were induced in those cell lines. The proportion of HLA-class II-positive cells or that of CEA-positive cells varied among the cell lines. Of the three antigens, the degree of HLA-class II expression paralleled that of growth inhibition by IFN-γ treatment. These results suggested that in various functions of IFN-γ against tumor cells, both the anti-proliferative effect and the induction of HLA-class II might be closely linked together in an intracellular signal transduction.

Original languageEnglish
Pages (from-to)767-769
Number of pages3
JournalBiotherapy
Volume12
Issue number5
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Interferons
Cell Differentiation
Cell Line
Neoplasms
HLA Antigens
Growth
Surface Antigens
Signal Transduction
Lung Neoplasms
Adenocarcinoma
Epithelial Cells

Keywords

  • CEA
  • HLA-class I
  • HLA-class II
  • IFN-γ
  • Lung cancer
  • Proliferation

ASJC Scopus subject areas

  • Cancer Research
  • Immunology and Allergy

Cite this

Yano, T., Kuninaka, S., Kanematsu, T., Uehara, T., Terazaki, Y., Fukuyama, Y., ... Ichinose, Y. (1998). Direct influences of interferon-γ on proliferation and differentiation of cancer cells. Biotherapy, 12(5), 767-769.

Direct influences of interferon-γ on proliferation and differentiation of cancer cells. / Yano, T.; Kuninaka, S.; Kanematsu, T.; Uehara, T.; Terazaki, Y.; Fukuyama, Y.; Yokoyama, H.; Asoh, H.; Ichinose, Y.

In: Biotherapy, Vol. 12, No. 5, 1998, p. 767-769.

Research output: Contribution to journalArticle

Yano, T, Kuninaka, S, Kanematsu, T, Uehara, T, Terazaki, Y, Fukuyama, Y, Yokoyama, H, Asoh, H & Ichinose, Y 1998, 'Direct influences of interferon-γ on proliferation and differentiation of cancer cells', Biotherapy, vol. 12, no. 5, pp. 767-769.
Yano T, Kuninaka S, Kanematsu T, Uehara T, Terazaki Y, Fukuyama Y et al. Direct influences of interferon-γ on proliferation and differentiation of cancer cells. Biotherapy. 1998;12(5):767-769.
Yano, T. ; Kuninaka, S. ; Kanematsu, T. ; Uehara, T. ; Terazaki, Y. ; Fukuyama, Y. ; Yokoyama, H. ; Asoh, H. ; Ichinose, Y. / Direct influences of interferon-γ on proliferation and differentiation of cancer cells. In: Biotherapy. 1998 ; Vol. 12, No. 5. pp. 767-769.
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