Direct influences of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6) on the proliferation and cell-surface antigen expression of cancer cells

Shinji Kuninaka, Tokujiro Yano, Hideki Yokoyama, Yasuro Fukuyama, Yasuhiro Terazaki, Tadashi Uehara, Takanori Kanematsu, Hiroshi Asoh, Yukito Ichinose

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Abstract

Pro-inflammatory cytokines, e.g. interleukin 1 (IL-1), tumour necrosis factor α (TNF-α), IL-6 produced by surgical intervention or non-specific immunotherapy may directly affect both the growth and the metastasis of tumour cells. It is therefore important to clarify the direct influence of pro-inflammatory cytokines on tumour cells in order to obtain a better knowledge of anti-tumour therapy. Four human lung cancer cell lines were used. The tumour cells were incubated for 72 h in the presence of various concentrations of IL-1β, TNF-α, or IL-6 and then the proliferative response was assessed by an MTT assay. After 14 days of culture with each pro-inflammatory cytokine, the cell-surface antigen expressions (HLA-class I, HLA-class II, CEA, sialyl Lewis(x)) were assessed by an immunocytochemical staining method. Among the various combinations of tumour cells (PC-9, PC-12, QG-56, QG-95) and cytokines IL-1β, TNF-α, IL-6), only TNF-α significantly exhibited an antiproliferative effect against PC-9 cells. However, various modulations of the cell-surface antigen expression by the cytokines were observed. The HLA-class I antigen expression of PC-9 was augmented by either TNF-α or IL-1β. Furthermore, IL-1β was able to induce CEA in PC-9, QG-56, and QG-95 cells while TNF-α was able to enhance the expression of sialyl Lewis(x) in QG-95 cells. Although the influence of pro-inflammatory cytokines on the growth of tumour cells was only slight, some modulations of the cell-surface antigen expression were notable. The augmentation of HLA-class I expression can thus improve the immunogenicity of tumour cells while the induction of CEA or sialyl Lewis(x) may therefore be associated with the promotion of metastasis.

Original languageEnglish
Pages (from-to)8-11
Number of pages4
JournalCytokine
Volume12
Issue number1
DOIs
Publication statusPublished - 2000 Jan
Externally publishedYes

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Keywords

  • Adhesion molecules
  • Human lymphocyte antigen (HLA)
  • Metastasis
  • Pro-inflammatory cytokine
  • Proliferation

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Kuninaka, S., Yano, T., Yokoyama, H., Fukuyama, Y., Terazaki, Y., Uehara, T., Kanematsu, T., Asoh, H., & Ichinose, Y. (2000). Direct influences of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6) on the proliferation and cell-surface antigen expression of cancer cells. Cytokine, 12(1), 8-11. https://doi.org/10.1006/cyto.1998.0504