Direct influences of proinflammatory cytokines (IL-1β, TNF-α, IL-6) on the proliferation and the cell-surface antigen expression of cancer cells

S. Kuninaka, T. Yano, T. Kanematsu, T. Uehara, Y. Terazaki, Y. Fukuyama, H. Yokoyama, H. Aso, Y. Ichinose

Research output: Contribution to journalArticle

Abstract

Background: The proinflammatory cytokines, i.e., IL-1, TNF-α and IL-6, produced by surgical intervention or non-specific immunotherapy, may directly affect both the growth and the metastasis of tumor cells. Therefore, it is important to clarify a direct influence of proinflammatory cytokines on tumor cells for better knowledge of anti-tumor therapy. Method: Four human lung cancer cell lines consisting of 2 squamous cell carcinoma and 2 adenocarcinoma were used. Tumor cells were incubated for 72 hours in the presence of various concentrations of each cytokine (IL-1β, TNF-α, IL-6), and the proliferative response was then assessed by MTT assay. After 14 days cultivation with those proinflammatory cytokines, cell-surface antigen expressions (HLA-class I, HLA-class II, CEA, sialyl Le(x)) were assessed by immunocytochemistry. Results: Among various combinations of tumor cells and cytokines, only TNF-α exhibited an antiproliferative effect against PC-9 cells. However, various modulations of cell-surface antigen expression by the cytokines were observed. The HLA-class I antigen expression of PC-9 was augmented by either TNF-α or IL-1β. Furthermore, IL-1β could induce CEA in QG-56, QG-95, and PC-9 cells, while TNF-α could enhance the expression of sialyl Le(x) in QG-95 cells. Conclusion: Although the influence of proinflammatory cytokines on the growth of tumor cells was rare, the modulation of cell-surface antigens was notable. The augmentation of HLA- class I expression can improve immunogenicity of tumor cells while the induction of CEA or sialyl Le(x) can be associated with the promotion of metastasis.

Original languageEnglish
Pages (from-to)615-617
Number of pages3
JournalBiotherapy
Volume12
Issue number5
Publication statusPublished - 1998
Externally publishedYes

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Surface Antigens
Interleukin-1
Interleukin-6
Cytokines
Neoplasms
Neoplasm Metastasis
Histocompatibility Antigens Class I
Growth
Immunotherapy
Squamous Cell Carcinoma
Lung Neoplasms
Adenocarcinoma
Immunohistochemistry
Cell Line

Keywords

  • Adhesion molecules
  • Human lymphocyte antigen (HLA)
  • Metastasis
  • Proinflammatory cytokine
  • Proliferation

ASJC Scopus subject areas

  • Cancer Research
  • Immunology and Allergy

Cite this

Kuninaka, S., Yano, T., Kanematsu, T., Uehara, T., Terazaki, Y., Fukuyama, Y., ... Ichinose, Y. (1998). Direct influences of proinflammatory cytokines (IL-1β, TNF-α, IL-6) on the proliferation and the cell-surface antigen expression of cancer cells. Biotherapy, 12(5), 615-617.

Direct influences of proinflammatory cytokines (IL-1β, TNF-α, IL-6) on the proliferation and the cell-surface antigen expression of cancer cells. / Kuninaka, S.; Yano, T.; Kanematsu, T.; Uehara, T.; Terazaki, Y.; Fukuyama, Y.; Yokoyama, H.; Aso, H.; Ichinose, Y.

In: Biotherapy, Vol. 12, No. 5, 1998, p. 615-617.

Research output: Contribution to journalArticle

Kuninaka, S, Yano, T, Kanematsu, T, Uehara, T, Terazaki, Y, Fukuyama, Y, Yokoyama, H, Aso, H & Ichinose, Y 1998, 'Direct influences of proinflammatory cytokines (IL-1β, TNF-α, IL-6) on the proliferation and the cell-surface antigen expression of cancer cells', Biotherapy, vol. 12, no. 5, pp. 615-617.
Kuninaka, S. ; Yano, T. ; Kanematsu, T. ; Uehara, T. ; Terazaki, Y. ; Fukuyama, Y. ; Yokoyama, H. ; Aso, H. ; Ichinose, Y. / Direct influences of proinflammatory cytokines (IL-1β, TNF-α, IL-6) on the proliferation and the cell-surface antigen expression of cancer cells. In: Biotherapy. 1998 ; Vol. 12, No. 5. pp. 615-617.
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AU - Kuninaka, S.

AU - Yano, T.

AU - Kanematsu, T.

AU - Uehara, T.

AU - Terazaki, Y.

AU - Fukuyama, Y.

AU - Yokoyama, H.

AU - Aso, H.

AU - Ichinose, Y.

PY - 1998

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