Direct observation of molecular transport between triplet cells via membrane proteins using a microfluidic system

Kosuke Inoue, Koki Kamiya, Yuta Abe, Toshihisa Osaki, Norihisa Miki, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This conference proceeding describes three cells trapping system in which the number and the arrangement of trapping cells can be controlled. First, we demonstrated three cells trapping in desiring order using the parylene slide device in which cells were desirably trapped by hydrostatic pressure when the parylene rail was slid on SU-8 structure and open. Next, we investigated the interaction between trapped cells by this devices using Connexin43 (Cx43) which formed gap junction in cell-to-cell. In this study, we successfully observed the calcein and cytored dye transfer from a donor cell containing fluorescent molecular to acceptor cells via Cx channel.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages820-821
Number of pages2
ISBN (Print)9780979806476
Publication statusPublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14/10/2614/10/30

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Keywords

  • Cell trapping
  • Connexin43
  • Gap junction

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Inoue, K., Kamiya, K., Abe, Y., Osaki, T., Miki, N., & Takeuchi, S. (2014). Direct observation of molecular transport between triplet cells via membrane proteins using a microfluidic system. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 820-821). Chemical and Biological Microsystems Society.