TY - GEN
T1 - Direct observation of molecular transport between triplet cells via membrane proteins using a microfluidic system
AU - Inoue, Kosuke
AU - Kamiya, Koki
AU - Abe, Yuta
AU - Osaki, Toshihisa
AU - Miki, Norihisa
AU - Takeuchi, Shoji
N1 - Publisher Copyright:
© 14CBMS.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2014
Y1 - 2014
N2 - This conference proceeding describes three cells trapping system in which the number and the arrangement of trapping cells can be controlled. First, we demonstrated three cells trapping in desiring order using the parylene slide device in which cells were desirably trapped by hydrostatic pressure when the parylene rail was slid on SU-8 structure and open. Next, we investigated the interaction between trapped cells by this devices using Connexin43 (Cx43) which formed gap junction in cell-to-cell. In this study, we successfully observed the calcein and cytored dye transfer from a donor cell containing fluorescent molecular to acceptor cells via Cx channel.
AB - This conference proceeding describes three cells trapping system in which the number and the arrangement of trapping cells can be controlled. First, we demonstrated three cells trapping in desiring order using the parylene slide device in which cells were desirably trapped by hydrostatic pressure when the parylene rail was slid on SU-8 structure and open. Next, we investigated the interaction between trapped cells by this devices using Connexin43 (Cx43) which formed gap junction in cell-to-cell. In this study, we successfully observed the calcein and cytored dye transfer from a donor cell containing fluorescent molecular to acceptor cells via Cx channel.
KW - Cell trapping
KW - Connexin43
KW - Gap junction
UR - http://www.scopus.com/inward/record.url?scp=84941662832&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941662832&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84941662832
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 820
EP - 821
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PB - Chemical and Biological Microsystems Society
T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Y2 - 26 October 2014 through 30 October 2014
ER -