Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry

Saira Hameed, Koji Ikegami, Eiji Sugiyama, Shoko Matsushita, Yoshishige Kimura, Takahiro Hayasaka, Yuki Sugiura, Noritaka Masaki, Michihiko Waki, Isao Ohta, Md Amir Hossen, Mitsutoshi Setou

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Abstract

A protocol for the direct analysis of the phospholipid composition in the whole body of adult soil nematode, Caenorhabditis elegans (C. elegans), was developed, which combined freeze-cracking of the exoskeletal cuticle and matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS). Biomolecules in the m/z range from 700 to 900 were more effectively detected in the freeze-cracked than from simple frozen adult nematode bodies. Different distribution of biomolecules was observed in a nematode body when the matrix was applied with a sublimation deposition method. The whole-body IMS technique was applied on genetically deficient mutant C. elegans to combine whole-body lipidomics and genetics, by comparing the fatty acid compositions, especially of the phosphatidylcholine (PC) species, between the wild-type and fat-1 mutants, which lack the gene encoding an n-3 fatty acid desaturase. A significant reduction of PC(20:5/20:5) and PC(20:4/20:5) and a marked increase of PC(20:4/20:4), PC(20:3/20:4), and PC(20:3/20:3) were detected in the fat-1 mutants in positive ion mode. In addition, phospholipid compositions other than PCs were analyzed in negative ion mode. A loss of a possible phosphatidylinositol (PI) with 18:0/20:5 and a compensative accumulation of putative PI(18:0/20:4) were detected in the fat-1 mutants. In conclusion, the whole-body MALDI-IMS technique is useful for the profiling of multiple biomolecules in C. elegans in both intra- and inter-individual levels.

Original languageEnglish
JournalAnalytical and Bioanalytical Chemistry
Volume407
Issue number25
DOIs
Publication statusPublished - 2017 Jan 1

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Keywords

  • Caenorhabditis elegans
  • Cuticle
  • Exoskeleton
  • Freeze-cracking
  • Matrix-assisted laser desorption/ionization-imaging mass spectrometry
  • Phosphatidylcholine
  • Phosphatidylinositol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Hameed, S., Ikegami, K., Sugiyama, E., Matsushita, S., Kimura, Y., Hayasaka, T., Sugiura, Y., Masaki, N., Waki, M., Ohta, I., Hossen, M. A., & Setou, M. (2017). Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry. Analytical and Bioanalytical Chemistry, 407(25). https://doi.org/10.1007/s00216-015-8932-7