Direct reprogramming of terminally differentiated B cells into erythroid lineage

Ken Sadahira, Yumi Fukuchi, Hiroyoshi Kunimono, Masatoshi Sakurai, Yasuo Ikeda, Shinichiro Okamoto, Hideaki Nakajima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Among various factors, GATA-1, SCL together with CCAAT/enhancer binding protein (C/EBP) α turned out to be a minimal set of factors that efficiently reprogrammed terminally differentiated mature B cells into erythroid lineage, as evidenced by colony forming assays and erythroid-specific gene expressions. This study sets an avenue to generate autologous erythrocytes from peripheral B cells.

Original languageEnglish
Pages (from-to)3645-3652
Number of pages8
JournalFEBS Letters
Volume586
Issue number20
DOIs
Publication statusPublished - 2012 Oct 19

Keywords

  • B cell
  • Erythroid
  • Lineage conversion
  • Reprogramming

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Sadahira, K., Fukuchi, Y., Kunimono, H., Sakurai, M., Ikeda, Y., Okamoto, S., & Nakajima, H. (2012). Direct reprogramming of terminally differentiated B cells into erythroid lineage. FEBS Letters, 586(20), 3645-3652. https://doi.org/10.1016/j.febslet.2012.08.019