Direct reprogramming of terminally differentiated B cells into erythroid lineage

Ken Sadahira, Yumi Fukuchi, Hiroyoshi Kunimono, Masatoshi Sakurai, Yasuo Ikeda, Shinichiro Okamoto, Hideaki Nakajima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Among various factors, GATA-1, SCL together with CCAAT/enhancer binding protein (C/EBP) α turned out to be a minimal set of factors that efficiently reprogrammed terminally differentiated mature B cells into erythroid lineage, as evidenced by colony forming assays and erythroid-specific gene expressions. This study sets an avenue to generate autologous erythrocytes from peripheral B cells.

Original languageEnglish
Pages (from-to)3645-3652
Number of pages8
JournalFEBS Letters
Volume586
Issue number20
DOIs
Publication statusPublished - 2012 Oct 19

Fingerprint

B-Lymphocytes
Cells
GATA Transcription Factors
CCAAT-Enhancer-Binding Proteins
Plasticity
Blood Cells
Transcription Factors
Erythrocytes
Gene expression
Gene Expression
Assays
Blood
Switches

Keywords

  • B cell
  • Erythroid
  • Lineage conversion
  • Reprogramming

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Sadahira, K., Fukuchi, Y., Kunimono, H., Sakurai, M., Ikeda, Y., Okamoto, S., & Nakajima, H. (2012). Direct reprogramming of terminally differentiated B cells into erythroid lineage. FEBS Letters, 586(20), 3645-3652. https://doi.org/10.1016/j.febslet.2012.08.019

Direct reprogramming of terminally differentiated B cells into erythroid lineage. / Sadahira, Ken; Fukuchi, Yumi; Kunimono, Hiroyoshi; Sakurai, Masatoshi; Ikeda, Yasuo; Okamoto, Shinichiro; Nakajima, Hideaki.

In: FEBS Letters, Vol. 586, No. 20, 19.10.2012, p. 3645-3652.

Research output: Contribution to journalArticle

Sadahira, K, Fukuchi, Y, Kunimono, H, Sakurai, M, Ikeda, Y, Okamoto, S & Nakajima, H 2012, 'Direct reprogramming of terminally differentiated B cells into erythroid lineage', FEBS Letters, vol. 586, no. 20, pp. 3645-3652. https://doi.org/10.1016/j.febslet.2012.08.019
Sadahira, Ken ; Fukuchi, Yumi ; Kunimono, Hiroyoshi ; Sakurai, Masatoshi ; Ikeda, Yasuo ; Okamoto, Shinichiro ; Nakajima, Hideaki. / Direct reprogramming of terminally differentiated B cells into erythroid lineage. In: FEBS Letters. 2012 ; Vol. 586, No. 20. pp. 3645-3652.
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