Direct transfer of tumor surface antigenic protein (TSAP) from tumor cell to liposome for making liposomal vaccine

Sunamoto Junzo, Noguchi Tetsurou, Sato Toshinori, Akiyoshi Kazunari, Shibata Ryujiro, Nakayama Ei-ichi, Shiku Hiroshi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

For the production of an effective liposomal vaccine, tumor surface antigenic proteins (TSAP) were directly transferred from BALB RVD leukemia cells to a lecithin liposome containing an artificial boundary lipid (DDPC, 1,2-dimyristoylamido-1,2-deoxyphospbatidylcholine). When DMPC (20 mol%)-DDPC (80 mol%) liposomes were employed the highest efficiency of membrane protein transfer was achieved. From studies of specific lectin-induced aggregation of the membrane protein-transferred liposome, saccharide moieties of proteins transferred were found to contain at least sialic acid, galactose, and mannose moieties. This membrane protein-transferred liposome showed significantly higher efficiency of macrophage uptake compared with the other two liposomes, namely no membrane protein-reconstituted liposome and liposome with proteins which were first extracted by butanol from turner cell and then reconstituted to liposome. Judging from results obtained, TSAP was considered to be certainly involved in membrane proteins transferred from the tumor cell to liposome. When mice were immunized by the TSAP-transferred liposome (liposomal vaccine) and subsequently challenged by BALB RVD, high CTL induction and excellent prevention of tumor growth were observed, as expected.

Original languageEnglish
Pages (from-to)143-153
Number of pages11
JournalJournal of Controlled Release
Volume20
Issue number2
DOIs
Publication statusPublished - 1992 Jun
Externally publishedYes

Keywords

  • Antigenic protein
  • Artificial boundary lipid
  • Leukemia
  • Liposomal vaccine
  • Liposome
  • Protein transfer

ASJC Scopus subject areas

  • Pharmaceutical Science

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