Direct visualization and quantitative analysis of water diffusion in complex biological tissues using CARS microscopy

Ying Chun Yu, Yoshiro Sohma, Shinichi Takimoto, Takayuki Miyauchi, Masato Yasui

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

To date, it has not been possible to measure microscopic diffusive water movements in epithelia and in the interstitial space of complex tissues and organs. Diffusive water movements are essential for life because they convey physiologically important small molecules, e.g. nutrients and signaling ligands throughout the extracellular space of complex tissues. Here we report the development of a novel method for the direct observation and quantitative analysis of water diffusion dynamics in a biologically organized tissue using Coherent Anti-Stokes Raman Scattering (CARS) microscopy. Using a computer simulation model to analyze the CARS O-H bond vibration data during H 2 O/D 2 O exchange in a 3D epithelial cyst, we succeeded in measuring the diffusive water permeability of the individual luminal and basolateral water pathways and also their response to hormonal stimulation. Our technique will be applicable to the measurement of diffusive water movements in other structurally complex and medically important tissues and organs.

Original languageEnglish
Article number2745
JournalScientific reports
Volume3
DOIs
Publication statusPublished - 2013

ASJC Scopus subject areas

  • General

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