Direct visualization of effects of endothelin on the renal microvasculature

Rodger Loutzenhiser, Murray Epstein, Koichi Hayashi, Charles Horton

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

The renal microvascular and hemodynamic actions of endothelin were assessed directly in isolated perfused hydronephrotic (HYD) and normal kidneys, respectively. In HYD kidneys endothelin was a potent vasoconstrictor of the afferent arteriole (AA), eliciting a threshold vasoconstrictor response at 0.01 nM (P < 0.05). At 0.1 and 0.3 nM, endothelin reduced AA diameter by 22 ± 6 (P < 0.025) and 41 ± 4% (P < 0.001), respectively. Furthermore, endothelin provoked oscillatory vasomotion in the AA. In contrast, endothelin had less effect on the efferent arteriole (EA), reducing EA diameter by only 7 ± 4 (P > 0.20) and 13 ± 4% (P < 0.05), at 0.1 and 0.3 nM, respectively. In normal kidneys endothelin elicited a long-lasting vasoconstriction with a dose dependency similar to that observed in the AA of HYD kidneys. Furthermore, endothelin reduced glomerular filtration rate (GFR) from 0.58 ± 0.04 to 0.09 ± 0.05 ml·min-1· g-1 (P < 0.001) in this model. Both the AA vasoconstriction and reduction in GFR were completely reversed by nifedipine. These findings indicate that endothelin is a potent renal vasoconstrictor that decreases GFR by a predominant vasoconstriction of the AA. Our observations are consistent with the postulate that endothelin elicits renal vasoconstriction via a mechanism involving dihydropyridine-sensitive calcium channels and that such calcium channels play a prominent role in the activation of the AA.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume258
Issue number1 27-1
Publication statusPublished - 1990

Fingerprint

Endothelins
Microvessels
Arterioles
Kidney
Vasoconstriction
Vasoconstrictor Agents
Glomerular Filtration Rate
Calcium Channels
Nifedipine
Hemodynamics

Keywords

  • Afferent arteriole
  • Calcium antagonists
  • Efferent arteriole
  • Endothelin i
  • Endothelium
  • Renal hemodynamics
  • Resistance arteries
  • Vasomotion

ASJC Scopus subject areas

  • Physiology

Cite this

Direct visualization of effects of endothelin on the renal microvasculature. / Loutzenhiser, Rodger; Epstein, Murray; Hayashi, Koichi; Horton, Charles.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 258, No. 1 27-1, 1990.

Research output: Contribution to journalArticle

Loutzenhiser, Rodger ; Epstein, Murray ; Hayashi, Koichi ; Horton, Charles. / Direct visualization of effects of endothelin on the renal microvasculature. In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1990 ; Vol. 258, No. 1 27-1.
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