Direct visualization of general anesthetic propofol on neurons by stimulated Raman scattering microscopy

Robert Oda, Jingwen Shou, Wenying Zhong, Yasuyuki Ozeki, Masato Yasui, Mutsuo Nuriya

Research output: Contribution to journalArticlepeer-review

Abstract

The consensus for the precise mechanism of action of general anesthetics is through allosteric interactions with GABA receptors in neurons. However, it has been speculated that these anesthetics may also interact with the plasma membrane on some level. Owing to the small size of anesthetics, direct visualization of these interactions is difficult to achieve. We demonstrate the ability to directly visualize a deuterated analog of propofol in living cells using stimulated Raman scattering (SRS) microscopy. Our findings support the theory that propofol is highly concentrated and interacts primarily through non-specific binding to the plasma membrane of neurons. Additionally, we show that SRS microscopy can be used to monitor the dynamics of propofol binding using real-time, live-cell imaging. The strategy used to visualize propofol can be applied to other small molecule drugs that have been previously invisible to traditional imaging techniques

Original languageEnglish
Article number103936
JournaliScience
Volume25
Issue number3
DOIs
Publication statusPublished - 2022 Mar 18

Keywords

  • Cell biology
  • Cellular neuroscience
  • Techniques in neuroscience

ASJC Scopus subject areas

  • General

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