Abstract
The polypeptide shell of the ferritin molecule has been imaged in water by atomic force microscopy (AFM). The central dip and the quaternary structure could be observed on the surface of the ferritin molecule anchored inhomogeneously in two dimensions. These structures observed in the AFM images are quite similar to the electron density map near the top of the apoferritin viewed down from a 4-fold axis structure reported previously (S. H. Banyard, D. K. Stammers, and P. M. Harrison. 1978. Nature (Lond.). 271:282-284). It has been achieved by introducing a 'self-screening effect' of the surface charges of the AFM sample (S. Ohnishi, M. Hara, T. Furuno, and H. Sasabe. 1992. Biophys. J. 63:1425-1431) and the specially sharpened stylus of AFM cantilever.
| Original language | English |
|---|---|
| Pages (from-to) | 573-577 |
| Number of pages | 5 |
| Journal | Biophysical Journal |
| Volume | 65 |
| Issue number | 2 |
| Publication status | Published - 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biophysics
Cite this
Direct visualization of polypeptide shell of ferritin molecule by atomic force microscopy. / Ohnishi, S.; Hara, M. H.; Furuno, Taiji; Okada, T.; Sasabe, H.
In: Biophysical Journal, Vol. 65, No. 2, 1993, p. 573-577.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct visualization of polypeptide shell of ferritin molecule by atomic force microscopy
AU - Ohnishi, S.
AU - Hara, M. H.
AU - Furuno, Taiji
AU - Okada, T.
AU - Sasabe, H.
PY - 1993
Y1 - 1993
N2 - The polypeptide shell of the ferritin molecule has been imaged in water by atomic force microscopy (AFM). The central dip and the quaternary structure could be observed on the surface of the ferritin molecule anchored inhomogeneously in two dimensions. These structures observed in the AFM images are quite similar to the electron density map near the top of the apoferritin viewed down from a 4-fold axis structure reported previously (S. H. Banyard, D. K. Stammers, and P. M. Harrison. 1978. Nature (Lond.). 271:282-284). It has been achieved by introducing a 'self-screening effect' of the surface charges of the AFM sample (S. Ohnishi, M. Hara, T. Furuno, and H. Sasabe. 1992. Biophys. J. 63:1425-1431) and the specially sharpened stylus of AFM cantilever.
AB - The polypeptide shell of the ferritin molecule has been imaged in water by atomic force microscopy (AFM). The central dip and the quaternary structure could be observed on the surface of the ferritin molecule anchored inhomogeneously in two dimensions. These structures observed in the AFM images are quite similar to the electron density map near the top of the apoferritin viewed down from a 4-fold axis structure reported previously (S. H. Banyard, D. K. Stammers, and P. M. Harrison. 1978. Nature (Lond.). 271:282-284). It has been achieved by introducing a 'self-screening effect' of the surface charges of the AFM sample (S. Ohnishi, M. Hara, T. Furuno, and H. Sasabe. 1992. Biophys. J. 63:1425-1431) and the specially sharpened stylus of AFM cantilever.
UR - http://www.scopus.com/inward/record.url?scp=0027301262&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027301262&partnerID=8YFLogxK
M3 - Article
C2 - 8218888
AN - SCOPUS:0027301262
VL - 65
SP - 573
EP - 577
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 2
ER -