Discontinuous total parenteral nutrition prevents postischemic mitochondrial dysfunction in rat liver

Nobuyuki Morikawa, Makoto Suematsu, Takanori Kyokane, Nobuhito Goda, Yusuke Kumamoto, Taro Okitsu, Yuzuru Ishimura, Masaki Kitajima

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Abstract

Although discontinuous total parenteral nutrition (d-TPN) has recently been favored for clinical use over continuous total parenteral nutrition (c- TPN) to ameliorate liver dysfunction, mechanisms for the protection against postoperative liver dysfunction remain unknown. This study aimed to examine differences in mitochondrial function in d-TPN-and c-TPN-pretreated livers during ischemia-reperfusion. Rat livers pretreated with d-TPN or c-TPN were perfused with Krebs-Ringer buffer and were exposed to 25% low-flow hypoxia followed by reperfusion. Intrahepatic mitochondrial membrane potential (ΔΨ) and cell viability were assessed by dual-color digital microfluorography using rhodamine 123 (Rh123) and propidium iodide (PI), respectively. In response to hypoxia, livers pretreated with c-TPN, d-TPN, and an ordinary chow diet exhibited a significant ΔΨ reduction among the entire lobules. Upon reperfusion, the regional ΔΨ values further decreased in the c-TPN liver, whereas those in the d-TPN-treated or chow-treated livers displayed a rapid recovery toward the control levels. The severity of cell injury did not differ among the groups, showing that the reperfusion-induced ΔΨ drop in the c-TPN-pretreated liver is not a consequence of cell injury. Differences in the ΔΨ drop among the groups appear to occur irrespective of those in the glycogen storage, because the livers undergoing d-TPN display a marked ΔΨ recovery even when reperfused at the end of a fasted state. These results indicate that c-TPN, but not d-TPN, jeopardizes mitochondrial re- energization and suggest that a circadian pattern of the TPN serves as a potentially beneficial strategy to reduce the risk of postischemic mitochondrial dysfunction in the liver.

Original languageEnglish
Pages (from-to)1289-1299
Number of pages11
JournalHepatology
Volume28
Issue number5
DOIs
Publication statusPublished - 1998 Nov 10

ASJC Scopus subject areas

  • Hepatology

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    Morikawa, N., Suematsu, M., Kyokane, T., Goda, N., Kumamoto, Y., Okitsu, T., Ishimura, Y., & Kitajima, M. (1998). Discontinuous total parenteral nutrition prevents postischemic mitochondrial dysfunction in rat liver. Hepatology, 28(5), 1289-1299. https://doi.org/10.1002/hep.510280518