Discovery, development, and testing of substrates and inhibitors of pp60(c-src)

R. J A Budde, J. S. McMurray, Hideyuki Saya, G. E. Gallick, V. A. Levin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Currently, there are no specific protein tyrosine kinase inhibitors available. This review summarizes our efforts to develop an active-site-directed inhibitor of pp60(c-src). Initial efforts are directed at determining substrate specificity with synthetic peptides and at developing a biological system to test the potential of pp60(c-src) inhibitors to effectively inhibit the growth of pp60(c-src) activated cell lines. To meet these goals, we have developed new methods for purifying recombinant pp60(c-src), assaying tyrosine kinase activity, synthesizing cyclic peptides, and generating random peptide libraries. In addition, we discuss the generation of potential artifacts while using polyhydroxy aromatic compounds as tyrosine kinase inhibitors.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalInternational Journal of Pharmacognosy
Volume33
Issue numberSUPPL.
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Proto-Oncogene Proteins pp60(c-src)
Peptide Library
Cyclic Peptides
Protein Kinase Inhibitors
Substrate Specificity
Protein-Tyrosine Kinases
Artifacts
Tyrosine
Catalytic Domain
Cell Line
Peptides
Growth

Keywords

  • Protein tyrosine kinase
  • Src
  • Synthetic peptides

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Budde, R. J. A., McMurray, J. S., Saya, H., Gallick, G. E., & Levin, V. A. (1995). Discovery, development, and testing of substrates and inhibitors of pp60(c-src). International Journal of Pharmacognosy, 33(SUPPL.), 27-34.

Discovery, development, and testing of substrates and inhibitors of pp60(c-src). / Budde, R. J A; McMurray, J. S.; Saya, Hideyuki; Gallick, G. E.; Levin, V. A.

In: International Journal of Pharmacognosy, Vol. 33, No. SUPPL., 1995, p. 27-34.

Research output: Contribution to journalArticle

Budde, RJA, McMurray, JS, Saya, H, Gallick, GE & Levin, VA 1995, 'Discovery, development, and testing of substrates and inhibitors of pp60(c-src)', International Journal of Pharmacognosy, vol. 33, no. SUPPL., pp. 27-34.
Budde, R. J A ; McMurray, J. S. ; Saya, Hideyuki ; Gallick, G. E. ; Levin, V. A. / Discovery, development, and testing of substrates and inhibitors of pp60(c-src). In: International Journal of Pharmacognosy. 1995 ; Vol. 33, No. SUPPL. pp. 27-34.
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