Discovery of a Small Molecule PDI Inhibitor That Inhibits Reduction of HIV-1 Envelope Glycoprotein gp120

Maola M G Khan, Siro Simizu, Ngit Shin Lai, Makoto Kawatani, Takeshi Shimizu, Hiroyuki Osada

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Protein disulfide isomerase (PDI) is a promiscuous protein with multifunctional properties. PDI mediates proper protein folding by oxidation or isomerization and disrupts disulfide bonds by reduction. The entry of HIV-1 into cells is facilitated by the PDI-catalyzed reductive cleavage of disulfide bonds in gp120. PDI is regarded as a potential drug target because of its reduction activity. We screened a chemical library of natural products for PDI-specific inhibitors in a high-throughput fashion and identified the natural compound juniferdin as the most potent inhibitor of PDI. Derivatives of juniferdin were synthesized, with compound 13 showing inhibitory activities comparable to those of juniferdin but reduced cytotoxicity. Both juniferdin and compound 13 inhibited PDI reductase activity in a dose-dependent manner, with IC50 values of 156 and 167 nM, respectively. Our results also indicated that juniferdin and compound 13 exert their inhibitory activities specifically on PDI but do not significantly inhibit homologues of this protein family. Moreover, we found that both compounds can inhibit PDI-mediated reduction of HIV-1 envelope glycoprotein gp120.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalACS Chemical Biology
Volume6
Issue number3
DOIs
Publication statusPublished - 2011 Mar 18

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HIV Envelope Protein gp120
Protein Disulfide-Isomerases
HIV-1
Glycoproteins
Molecules
Disulfides
Small Molecule Libraries
Protein folding
Protein Folding
Cytotoxicity
Isomerization
Biological Products
Inhibitory Concentration 50
Oxidoreductases
Proteins
Throughput
juniferdin
Derivatives

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

Cite this

Discovery of a Small Molecule PDI Inhibitor That Inhibits Reduction of HIV-1 Envelope Glycoprotein gp120. / Khan, Maola M G; Simizu, Siro; Lai, Ngit Shin; Kawatani, Makoto; Shimizu, Takeshi; Osada, Hiroyuki.

In: ACS Chemical Biology, Vol. 6, No. 3, 18.03.2011, p. 245-251.

Research output: Contribution to journalArticle

Khan, Maola M G ; Simizu, Siro ; Lai, Ngit Shin ; Kawatani, Makoto ; Shimizu, Takeshi ; Osada, Hiroyuki. / Discovery of a Small Molecule PDI Inhibitor That Inhibits Reduction of HIV-1 Envelope Glycoprotein gp120. In: ACS Chemical Biology. 2011 ; Vol. 6, No. 3. pp. 245-251.
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