Abstract
Cell sorting is an important technology that is widely used for medical diagnosis in hospitals and cell engineering research. Among cell sorting technology, dielectrophoresis (DEP) is one of the most promising approaches for manipulating and separating biological particles because this phenomena requires no labeling procedure with a fluorescent dye or magnetic beads. In this study, we developed a precise cell sorting system by evaluating the DEP force with a liquid flow system. The DEP forces acting on a cell or polystyrene microbead (cell simulant) were characterized using a microfluidic chamber containing an electrode-array and fluid-induced shear forces. On the basis of this characterization, separation of the cells and microbeads was performed using our novel DEP cell sorting system. As a result, the living cells were trapped by the DEP force on the electrode arrays, whereas the beads passed the electrode array. In conclusion, the DEP force combined with fluid-induced shear force could separate the living cells from cell simulants.
| Original language | English |
|---|---|
| Pages (from-to) | 42-50 |
| Number of pages | 9 |
| Journal | Journal of Biorheology |
| Volume | 29 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2015 |
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Keywords
- Cell sorting
- Dielectrophoresis
- MEMS
- Microfluidics
- Wall shear force
ASJC Scopus subject areas
- Mechanics of Materials
- Materials Science(all)
- Mechanical Engineering
Cite this
Discrimination methodology of living-cells and microbeads using dielectrophoresis and fluid-induced shear force. / Ojima, Yuta; Miyata, Shogo.
In: Journal of Biorheology, Vol. 29, No. 1, 2015, p. 42-50.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Discrimination methodology of living-cells and microbeads using dielectrophoresis and fluid-induced shear force
AU - Ojima, Yuta
AU - Miyata, Shogo
PY - 2015
Y1 - 2015
N2 - Cell sorting is an important technology that is widely used for medical diagnosis in hospitals and cell engineering research. Among cell sorting technology, dielectrophoresis (DEP) is one of the most promising approaches for manipulating and separating biological particles because this phenomena requires no labeling procedure with a fluorescent dye or magnetic beads. In this study, we developed a precise cell sorting system by evaluating the DEP force with a liquid flow system. The DEP forces acting on a cell or polystyrene microbead (cell simulant) were characterized using a microfluidic chamber containing an electrode-array and fluid-induced shear forces. On the basis of this characterization, separation of the cells and microbeads was performed using our novel DEP cell sorting system. As a result, the living cells were trapped by the DEP force on the electrode arrays, whereas the beads passed the electrode array. In conclusion, the DEP force combined with fluid-induced shear force could separate the living cells from cell simulants.
AB - Cell sorting is an important technology that is widely used for medical diagnosis in hospitals and cell engineering research. Among cell sorting technology, dielectrophoresis (DEP) is one of the most promising approaches for manipulating and separating biological particles because this phenomena requires no labeling procedure with a fluorescent dye or magnetic beads. In this study, we developed a precise cell sorting system by evaluating the DEP force with a liquid flow system. The DEP forces acting on a cell or polystyrene microbead (cell simulant) were characterized using a microfluidic chamber containing an electrode-array and fluid-induced shear forces. On the basis of this characterization, separation of the cells and microbeads was performed using our novel DEP cell sorting system. As a result, the living cells were trapped by the DEP force on the electrode arrays, whereas the beads passed the electrode array. In conclusion, the DEP force combined with fluid-induced shear force could separate the living cells from cell simulants.
KW - Cell sorting
KW - Dielectrophoresis
KW - MEMS
KW - Microfluidics
KW - Wall shear force
UR - http://www.scopus.com/inward/record.url?scp=84978630418&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84978630418&partnerID=8YFLogxK
U2 - 10.17106/jbr.29.42
DO - 10.17106/jbr.29.42
M3 - Article
VL - 29
SP - 42
EP - 50
JO - Journal of Biorheology
JF - Journal of Biorheology
SN - 1867-0466
IS - 1
ER -