Disorganized Steroidogenesis in Adrenocortical Carcinoma, a Case Study

Toyoyoshi Uchida, Koshiro Nishimoto, Yuki Fukumura, Miki Asahina, Hiromasa Goto, Yui Kawano, Fumitaka Shimizu, Akira Tsujimura, Tsugio Seki, Kuniaki Mukai, Yasuaki Kabe, Makoto Suematsu, Celso E. Gomez-Sanchez, Takashi Yao, Shigeo Horie, Hirotaka Watada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Most adrenocortical carcinomas (ACCs) produce excessive amounts of steroid hormones including aldosterone, cortisol, and steroid precursors. However, aldosterone- and cortisol-producing cells in ACCs have not yet been immunohistochemically described. We present a case of ACC causing mild primary aldosteronism and subclinical Cushing’s syndrome. Removal of the tumor cured both conditions. In order to examine the expression patterns of the steroidogenic enzymes responsible for adrenocortical hormone production, 10 tumor portions were immunohistochemically analyzed for aldosterone synthase (CYP11B2), 11β-hydroxylase (CYP11B1, cortisol-synthesizing enzyme), 3β-hydroxysteroid dehydrogenase (3βHSD, upstream enzyme for both CYP11B2 and CYP11B1), and 17α-hydroxylase/C17-20 lyase (CYP17, upstream enzyme for CYP11B1, but not for CYP11B1). CYP11B2, CYP11B1, and 3βHSD were expressed sporadically, and their expression patterns varied significantly among the different tumor portions examined. The expression of these enzymes was random and not associated with each other. CYP17 was expressed throughout the tumor, even in CYP11B2-positive cells. Small tumor cell populations were aldosterone- or cortisol-producing cells, as judged by 3βHSD coinciding with either CYP11B2 or CYP11B1, respectively. These results suggest that the tumor produced limited amounts of aldosterone and cortisol due to the lack of the coordinated expression of steroidogenic enzymes, which led to mild clinical expression in this case. We delineated the expression patterns of steroidogenic enzymes in ACC. The coordinated expression of steroidogenic enzymes in normal and adenoma cells was disturbed in ACC cells, resulting in the inefficient production of steroid hormones in relation to the large tumor volume.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalEndocrine Pathology
DOIs
Publication statusAccepted/In press - 2016 Jul 18

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Adrenocortical Carcinoma
Steroid 11-beta-Hydroxylase
Cytochrome P-450 CYP11B2
Hydrocortisone
Aldosterone
Enzymes
Neoplasms
Steroids
Hormones
Mixed Function Oxygenases
3-Hydroxysteroid Dehydrogenases
Steroid 17-alpha-Hydroxylase
Hyperaldosteronism
Lyases
Cushing Syndrome
Tumor Burden
Adenoma

Keywords

  • 11β-hydroxylase
  • Adrenocortical carcinoma
  • Aldosterone synthase
  • Primary aldosteronism
  • Subclinical Cushing’s syndrome

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Uchida, T., Nishimoto, K., Fukumura, Y., Asahina, M., Goto, H., Kawano, Y., ... Watada, H. (Accepted/In press). Disorganized Steroidogenesis in Adrenocortical Carcinoma, a Case Study. Endocrine Pathology, 1-9. https://doi.org/10.1007/s12022-016-9441-8

Disorganized Steroidogenesis in Adrenocortical Carcinoma, a Case Study. / Uchida, Toyoyoshi; Nishimoto, Koshiro; Fukumura, Yuki; Asahina, Miki; Goto, Hiromasa; Kawano, Yui; Shimizu, Fumitaka; Tsujimura, Akira; Seki, Tsugio; Mukai, Kuniaki; Kabe, Yasuaki; Suematsu, Makoto; Gomez-Sanchez, Celso E.; Yao, Takashi; Horie, Shigeo; Watada, Hirotaka.

In: Endocrine Pathology, 18.07.2016, p. 1-9.

Research output: Contribution to journalArticle

Uchida, T, Nishimoto, K, Fukumura, Y, Asahina, M, Goto, H, Kawano, Y, Shimizu, F, Tsujimura, A, Seki, T, Mukai, K, Kabe, Y, Suematsu, M, Gomez-Sanchez, CE, Yao, T, Horie, S & Watada, H 2016, 'Disorganized Steroidogenesis in Adrenocortical Carcinoma, a Case Study', Endocrine Pathology, pp. 1-9. https://doi.org/10.1007/s12022-016-9441-8
Uchida T, Nishimoto K, Fukumura Y, Asahina M, Goto H, Kawano Y et al. Disorganized Steroidogenesis in Adrenocortical Carcinoma, a Case Study. Endocrine Pathology. 2016 Jul 18;1-9. https://doi.org/10.1007/s12022-016-9441-8
Uchida, Toyoyoshi ; Nishimoto, Koshiro ; Fukumura, Yuki ; Asahina, Miki ; Goto, Hiromasa ; Kawano, Yui ; Shimizu, Fumitaka ; Tsujimura, Akira ; Seki, Tsugio ; Mukai, Kuniaki ; Kabe, Yasuaki ; Suematsu, Makoto ; Gomez-Sanchez, Celso E. ; Yao, Takashi ; Horie, Shigeo ; Watada, Hirotaka. / Disorganized Steroidogenesis in Adrenocortical Carcinoma, a Case Study. In: Endocrine Pathology. 2016 ; pp. 1-9.
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