Disposable electrochemical biosensor based on surface-modified screen-printed electrodes for organophosphorus pesticide analysis

Quoc Trung Hua, Nipapan Ruecha, Yuki Hiruta, Daniel Citterio

Research output: Contribution to journalArticle

Abstract

A disposable enzymatic electrochemical biosensor for amperometric organophosphorus pesticide determination based on a screen-printed carbon electrode (SPCE) platform was successfully developed. The working electrode consists of acetylcholine esterase (AChE) immobilized onto the surface of multi-walled carbon nanotube (MWCNT), chitosan (CS) and gold nanoparticle (AuNP)-modified SPCE. Ferricyanide was used as a redox mediator in solution. Two linear dynamic response ranges from 0.01 to 10 μg L-1 and from 10 to 100 μg L-1 were found for paraoxon-ethyl, with a detection limit of 0.03 μg L-1 (calculated as the amount of pesticide resulting in 10% of enzyme inhibition). High sensitivity was achieved due to a synergistic effect of AuNPs and MWCNTs deposited on the SPCE surface. Moreover, 83% of enzyme activity was retained after a dry storage period of 49 days (4 °C). Finally, the results of the electrochemical biosensor for paraoxon-ethyl-spiked spinach sample analysis showed a good agreement with those obtained from conventional HPLC.

Original languageEnglish
Pages (from-to)3439-3445
Number of pages7
JournalAnalytical Methods
Volume11
Issue number27
DOIs
Publication statusPublished - 2019 Jul 21

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Pesticides
Biosensors
Electrodes
Carbon
Enzyme inhibition
Carbon Nanotubes
Chitosan
Enzyme activity
Esterases
Gold
Acetylcholine
Dynamic response
Carbon nanotubes
Nanoparticles
ethylparaoxon

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

Cite this

Disposable electrochemical biosensor based on surface-modified screen-printed electrodes for organophosphorus pesticide analysis. / Hua, Quoc Trung; Ruecha, Nipapan; Hiruta, Yuki; Citterio, Daniel.

In: Analytical Methods, Vol. 11, No. 27, 21.07.2019, p. 3439-3445.

Research output: Contribution to journalArticle

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