Disruption of AMPA receptor GluR2 clusters following long-term depression induction in cerebellar Purkinje neurons

Shinji Matsuda, Thomas Launey, Sumiko Mikawa, Hirokazu Hirai

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

Cerebellar long-term depression (LTD) is thought to play an important role in certain types of motor learning. However, the molecular mechanisms underlying this event have not been clarified. Here, using cultured Purkinje cells, we show that stimulations inducing cerebellar LTD cause phosphorylation of Ser880 in the intracellular C-terminal domain of the AMPA receptor subunit GluR2. This phosphorylation is accompanied by both a reduction in the affinity of GluR2 to glutamate receptor interacting protein (GRIP), a molecule known to be critical for AMPA receptor clustering, and a significant disruption of postsynaptic GluR2 clusters. Moreover, GluR2 protein released from GRIP is shown to be internalized. These results suggest that the dissociation of postsynaptic GluR2 clusters and subsequent internalization of the receptor protein, initiated by the phosphorylation of Ser880, are the mechanisms underlying the induction of cerebellar LTD.

Original languageEnglish
Pages (from-to)2765-2774
Number of pages10
JournalEMBO Journal
Volume19
Issue number12
Publication statusPublished - 2000 Jun 15

Fingerprint

Phosphorylation
AMPA Receptors
Purkinje Cells
Receptor-Interacting Protein Serine-Threonine Kinases
Neurons
Glutamate Receptors
Depression
Cluster Analysis
Cultured Cells
Proteins
Learning
Molecules

Keywords

  • AMPA receptor
  • Cluster
  • GluR2
  • LTD
  • Purkinje cell

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Disruption of AMPA receptor GluR2 clusters following long-term depression induction in cerebellar Purkinje neurons. / Matsuda, Shinji; Launey, Thomas; Mikawa, Sumiko; Hirai, Hirokazu.

In: EMBO Journal, Vol. 19, No. 12, 15.06.2000, p. 2765-2774.

Research output: Contribution to journalArticle

Matsuda, S, Launey, T, Mikawa, S & Hirai, H 2000, 'Disruption of AMPA receptor GluR2 clusters following long-term depression induction in cerebellar Purkinje neurons', EMBO Journal, vol. 19, no. 12, pp. 2765-2774.
Matsuda, Shinji ; Launey, Thomas ; Mikawa, Sumiko ; Hirai, Hirokazu. / Disruption of AMPA receptor GluR2 clusters following long-term depression induction in cerebellar Purkinje neurons. In: EMBO Journal. 2000 ; Vol. 19, No. 12. pp. 2765-2774.
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