Disruption of aquaporin-11 produces polycystic kidneys following vacuolization of the proximal tubule

Yoshiyuki Morishita, Toshiyuki Matsuzaki, Mariko Chikuma, Ayaka Andoo, Mariko Shimono, Asako Matsuki, Katsuki Kobayashi, Masahiro Ikeda, Tadashi Yamamoto, Alan Verkman, Eiji Kusano, Shigeo Ookawara, Kuniaki Takata, Sei Sasaki, Kenichi Ishibashi

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Aquaporin-11 (AQP11) has been identified with unusual pore-forming NPA (asparagine-proline-alanine) boxes, but its function is unknown. We investigated its potential contribution to the kidney. Immunohistochemistry revealed that AQP11 was localized intracellularly in the proximal tubule. When AQP11 was transfected in CHO-K1 cells, it was localized in intracellular organelles. AQP11-null mice were generated; these mice exhibited vacuolization and cyst formation of the proximal tubule. AQP11-null mice were born normally but died before weaning due to advanced renal failure with polycystic kidneys, in which cysts occupied the whole cortex. Remarkably, cyst epithelia contained vacuoles. These vacuoles were present in the proximal tubules of newborn mice. In 3-week-old mice, these tubules contained multiple cysts. Primary cultured cells of the proximal tubule revealed an endosomal acidification defect in AQP11-null mice. These data demonstrate that AQP11 is essential for the proximal tubular function. AQP11-null mice are a novel model for polycystic kidney diseases and will provide a new mechanism for cystogenesis.

Original languageEnglish
Pages (from-to)7770-7779
Number of pages10
JournalMolecular and Cellular Biology
Volume25
Issue number17
DOIs
Publication statusPublished - 2005 Sep
Externally publishedYes

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Polycystic Kidney Diseases
Aquaporins
Cysts
Vacuoles
CHO Cells
Weaning
Organelles
Renal Insufficiency
Cultured Cells
Epithelium
Immunohistochemistry
Kidney

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Disruption of aquaporin-11 produces polycystic kidneys following vacuolization of the proximal tubule. / Morishita, Yoshiyuki; Matsuzaki, Toshiyuki; Chikuma, Mariko; Andoo, Ayaka; Shimono, Mariko; Matsuki, Asako; Kobayashi, Katsuki; Ikeda, Masahiro; Yamamoto, Tadashi; Verkman, Alan; Kusano, Eiji; Ookawara, Shigeo; Takata, Kuniaki; Sasaki, Sei; Ishibashi, Kenichi.

In: Molecular and Cellular Biology, Vol. 25, No. 17, 09.2005, p. 7770-7779.

Research output: Contribution to journalArticle

Morishita, Y, Matsuzaki, T, Chikuma, M, Andoo, A, Shimono, M, Matsuki, A, Kobayashi, K, Ikeda, M, Yamamoto, T, Verkman, A, Kusano, E, Ookawara, S, Takata, K, Sasaki, S & Ishibashi, K 2005, 'Disruption of aquaporin-11 produces polycystic kidneys following vacuolization of the proximal tubule', Molecular and Cellular Biology, vol. 25, no. 17, pp. 7770-7779. https://doi.org/10.1128/MCB.25.17.7770-7779.2005
Morishita, Yoshiyuki ; Matsuzaki, Toshiyuki ; Chikuma, Mariko ; Andoo, Ayaka ; Shimono, Mariko ; Matsuki, Asako ; Kobayashi, Katsuki ; Ikeda, Masahiro ; Yamamoto, Tadashi ; Verkman, Alan ; Kusano, Eiji ; Ookawara, Shigeo ; Takata, Kuniaki ; Sasaki, Sei ; Ishibashi, Kenichi. / Disruption of aquaporin-11 produces polycystic kidneys following vacuolization of the proximal tubule. In: Molecular and Cellular Biology. 2005 ; Vol. 25, No. 17. pp. 7770-7779.
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