Disruption of Dicer1 Induces Dysregulated Fetal Gene Expression and Promotes Hepatocarcinogenesis

Shigeki Sekine, Reiko Ogawa, Rie Ito, Nobuyoshi Hiraoka, Michael T. McManus, Yae Kanai, Matthias Hebrok

Research output: Contribution to journalArticle

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Abstract

Background & Aims: Growing evidence suggests that microRNAs coordinate various biological processes in the liver. We describe experiments to address the physiologic roles of these new regulators of gene expression in the liver that are as of yet largely undefined. Methods: We disrupted Dicer, an enzyme essential for the processing of microRNAs, in hepatocytes using a conditional knockout mouse model to elucidate the consequences of loss of microRNAs. Results: The conditional knockout mouse livers showed the efficient disruption of Dicer1 at 3 weeks after birth. This resulted in prominent steatosis and the depletion of glycogen storage. Dicer1-deficient liver exhibited increased growth-promoting gene expression and the robust expression of fetal stage-specific genes. The consequence of Dicer elimination included both increased hepatocyte proliferation and overwhelming apoptosis. Over time, Dicer1-expressing wild-type hepatocytes that had escaped Cre-mediated recombination progressively repopulated the entire liver. Unexpectedly, however, two thirds of the mutant mice spontaneously developed hepatocellular carcinomas derived from residual Dicer1-deficient hepatocytes at 1 year of age. Conclusions: Dicer and microRNAs have critical roles in hepatocyte survival, metabolism, developmental gene regulation, and tumor suppression in the liver. Loss of Dicer primarily impairs hepatocyte survival but can promote hepatocarcinogenesis in cooperation with additional oncogenic stimuli.

Original languageEnglish
JournalGastroenterology
Volume136
Issue number7
DOIs
Publication statusPublished - 2009 Jun
Externally publishedYes

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Hepatocytes
MicroRNAs
Gene Expression
Liver
Knockout Mice
Ribonuclease III
Developmental Genes
Biological Phenomena
Regulator Genes
Glycogen
Genetic Recombination
Hepatocellular Carcinoma
Parturition
Apoptosis
Growth
Genes
Neoplasms

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Disruption of Dicer1 Induces Dysregulated Fetal Gene Expression and Promotes Hepatocarcinogenesis. / Sekine, Shigeki; Ogawa, Reiko; Ito, Rie; Hiraoka, Nobuyoshi; McManus, Michael T.; Kanai, Yae; Hebrok, Matthias.

In: Gastroenterology, Vol. 136, No. 7, 06.2009.

Research output: Contribution to journalArticle

Sekine, Shigeki ; Ogawa, Reiko ; Ito, Rie ; Hiraoka, Nobuyoshi ; McManus, Michael T. ; Kanai, Yae ; Hebrok, Matthias. / Disruption of Dicer1 Induces Dysregulated Fetal Gene Expression and Promotes Hepatocarcinogenesis. In: Gastroenterology. 2009 ; Vol. 136, No. 7.
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