Disruption of Sept6, a fusion partner gene of MLL, does not affect ontogeny, leukemogenesis induced by MLL-SEPT6, or phenotype induced by the loss of Sept4

Ryoichi Ono, Masafumi Ihara, Hideaki Nakajima, Katsutoshi Ozaki, Yuki Kataoka-Fujiwara, Tomohiko Taki, Koh Ichi Nagata, Masaki Inagaki, Nobuaki Yoshida, Toshio Kitamura, Yasuhide Hayashi, Makoto Kinoshita, Tetsuya Nosaka

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Septins are evolutionarily conserved GTP-binding proteins that can heteropolymerize into filaments. Recent studies have revealed that septins are involved in not only diverse normal cellular processes but also the pathogenesis of various diseases, including cancer. SEPT6 is ubiquitously expressed in tissues and one of the fusion partner genes of MLL in the 11q23 translocations implicated in acute leukemia. However, the roles of this septin in vivo remain elusive. We have developed Sept6-deficient mice that exhibited neither gross abnormalities, changes in cytokinesis, nor spontaneous malignancy. Sept6 deficiency did not cause any quantitative changes in any of the septins evaluated in this study, nor did it cause any additional changes in the Sept4-deficient mice. Even the depletion of Sept11, a close homolog of Sept6, did not affect the Sept6-null cells in vitro, thus implying a high degree of redundancy in the septin system. Furthermore, a loss of Sept6 did not alter the phenotype of myeloproliferative disease induced by MLL-SEPT6, thus suggesting that Sept6 does not function as a tumor suppressor. To our knowledge, this is the first report demonstrating that a disruption of the translocation partner gene of MLL in 11q23 translocation does not contribute to leukemogenesis by the MLL fusion gene.

Original languageEnglish
Pages (from-to)10965-10978
Number of pages14
JournalMolecular and cellular biology
Volume25
Issue number24
DOIs
Publication statusPublished - 2005 Dec 1

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Disruption of Sept6, a fusion partner gene of MLL, does not affect ontogeny, leukemogenesis induced by MLL-SEPT6, or phenotype induced by the loss of Sept4'. Together they form a unique fingerprint.

  • Cite this

    Ono, R., Ihara, M., Nakajima, H., Ozaki, K., Kataoka-Fujiwara, Y., Taki, T., Nagata, K. I., Inagaki, M., Yoshida, N., Kitamura, T., Hayashi, Y., Kinoshita, M., & Nosaka, T. (2005). Disruption of Sept6, a fusion partner gene of MLL, does not affect ontogeny, leukemogenesis induced by MLL-SEPT6, or phenotype induced by the loss of Sept4. Molecular and cellular biology, 25(24), 10965-10978. https://doi.org/10.1128/MCB.25.24.10965-10978.2005