Distal colorectal cancers with microsatellite instability (MSI) display distinct gene expression profiles that are different from proximal MSI cancers

Toshiaki Watanabe, Takashi Kobunai, Etsuko Toda, Yoko Yamamoto, Takamitsu Kanazawa, Yoshihiro Kazama, Junichiro Tanaka, Toshiaki Tanaka, Tsuyosi Konishi, Yoshihiro Okayama, Yoshikazu Sugimoto, Toshinori Oka, Shin Sasaki, Tetsuichiro Muto, Hirokazu Nagawa

Research output: Contribution to journalArticle

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Abstract

Promoter methylation of the mismatch repair gene plays a key role in sporadic microsatellite instability (MSI) colorectal cancers. However, promoter methylation often occurs in proximal colon cancers, and molecular phenotypes underlying MSI cancers in distal colon have not been fully clarified. Our goal was to clarify the difference between MSI and microsatellite stability (MSS) cancers and, furthermore, to determine distinct characteristics of proximal and distal MSI cancers. By DNA microarray analysis of 84 cancers (33 MSI and 51 MSS), we identified discriminating genes (177 probe sets), which predicted MSI status with a high accuracy rate (97.6%). These genes were related to phenotypic characteristics of MSI cancers. Next, we identified 24 probe sets that were differentially expressed in proximal and distal MSI cancers. These genes included promoter methylation-mediated genes, whose expression was significantly down-regulated in proximal MSI cancers. Among discriminating genes between MSI and MSS, nine methylation-mediated genes showed down-regulation in MSI cancers. Of these, 7 (77.8%) showed down-regulation in proximal MSI cancers. Furthermore, methylation-specific PCR confirmed that frequency of hMLH1 promoter methylation was significantly higher in proximal MSI cancers (P = 0.0317). These results suggested that there is a difference between proximal and distal MSI cancers in methylation-mediated influence on gene silencing. In conclusion, using DNA microarray, we could distinguish MSI and MSS cancers. We also showed distinct characteristics of proximal and distal MSI cancers. The inactivation form of hMLH, per se, differed between proximal and distal MSI cancers. These results suggested that distal MSI cancers constitute a distinct subgroup of sporadic MSI cancers.

Original languageEnglish
Pages (from-to)9804-9808
Number of pages5
JournalCancer Research
Volume66
Issue number20
DOIs
Publication statusPublished - 2006 Oct 15
Externally publishedYes

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Microsatellite Instability
Transcriptome
Colorectal Neoplasms
Neoplasms
Methylation
Microsatellite Repeats
Genes
Oligonucleotide Array Sequence Analysis
Down-Regulation
DNA Mismatch Repair

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Distal colorectal cancers with microsatellite instability (MSI) display distinct gene expression profiles that are different from proximal MSI cancers. / Watanabe, Toshiaki; Kobunai, Takashi; Toda, Etsuko; Yamamoto, Yoko; Kanazawa, Takamitsu; Kazama, Yoshihiro; Tanaka, Junichiro; Tanaka, Toshiaki; Konishi, Tsuyosi; Okayama, Yoshihiro; Sugimoto, Yoshikazu; Oka, Toshinori; Sasaki, Shin; Muto, Tetsuichiro; Nagawa, Hirokazu.

In: Cancer Research, Vol. 66, No. 20, 15.10.2006, p. 9804-9808.

Research output: Contribution to journalArticle

Watanabe, T, Kobunai, T, Toda, E, Yamamoto, Y, Kanazawa, T, Kazama, Y, Tanaka, J, Tanaka, T, Konishi, T, Okayama, Y, Sugimoto, Y, Oka, T, Sasaki, S, Muto, T & Nagawa, H 2006, 'Distal colorectal cancers with microsatellite instability (MSI) display distinct gene expression profiles that are different from proximal MSI cancers', Cancer Research, vol. 66, no. 20, pp. 9804-9808. https://doi.org/10.1158/0008-5472.CAN-06-1163
Watanabe, Toshiaki ; Kobunai, Takashi ; Toda, Etsuko ; Yamamoto, Yoko ; Kanazawa, Takamitsu ; Kazama, Yoshihiro ; Tanaka, Junichiro ; Tanaka, Toshiaki ; Konishi, Tsuyosi ; Okayama, Yoshihiro ; Sugimoto, Yoshikazu ; Oka, Toshinori ; Sasaki, Shin ; Muto, Tetsuichiro ; Nagawa, Hirokazu. / Distal colorectal cancers with microsatellite instability (MSI) display distinct gene expression profiles that are different from proximal MSI cancers. In: Cancer Research. 2006 ; Vol. 66, No. 20. pp. 9804-9808.
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T1 - Distal colorectal cancers with microsatellite instability (MSI) display distinct gene expression profiles that are different from proximal MSI cancers

AU - Watanabe, Toshiaki

AU - Kobunai, Takashi

AU - Toda, Etsuko

AU - Yamamoto, Yoko

AU - Kanazawa, Takamitsu

AU - Kazama, Yoshihiro

AU - Tanaka, Junichiro

AU - Tanaka, Toshiaki

AU - Konishi, Tsuyosi

AU - Okayama, Yoshihiro

AU - Sugimoto, Yoshikazu

AU - Oka, Toshinori

AU - Sasaki, Shin

AU - Muto, Tetsuichiro

AU - Nagawa, Hirokazu

PY - 2006/10/15

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N2 - Promoter methylation of the mismatch repair gene plays a key role in sporadic microsatellite instability (MSI) colorectal cancers. However, promoter methylation often occurs in proximal colon cancers, and molecular phenotypes underlying MSI cancers in distal colon have not been fully clarified. Our goal was to clarify the difference between MSI and microsatellite stability (MSS) cancers and, furthermore, to determine distinct characteristics of proximal and distal MSI cancers. By DNA microarray analysis of 84 cancers (33 MSI and 51 MSS), we identified discriminating genes (177 probe sets), which predicted MSI status with a high accuracy rate (97.6%). These genes were related to phenotypic characteristics of MSI cancers. Next, we identified 24 probe sets that were differentially expressed in proximal and distal MSI cancers. These genes included promoter methylation-mediated genes, whose expression was significantly down-regulated in proximal MSI cancers. Among discriminating genes between MSI and MSS, nine methylation-mediated genes showed down-regulation in MSI cancers. Of these, 7 (77.8%) showed down-regulation in proximal MSI cancers. Furthermore, methylation-specific PCR confirmed that frequency of hMLH1 promoter methylation was significantly higher in proximal MSI cancers (P = 0.0317). These results suggested that there is a difference between proximal and distal MSI cancers in methylation-mediated influence on gene silencing. In conclusion, using DNA microarray, we could distinguish MSI and MSS cancers. We also showed distinct characteristics of proximal and distal MSI cancers. The inactivation form of hMLH, per se, differed between proximal and distal MSI cancers. These results suggested that distal MSI cancers constitute a distinct subgroup of sporadic MSI cancers.

AB - Promoter methylation of the mismatch repair gene plays a key role in sporadic microsatellite instability (MSI) colorectal cancers. However, promoter methylation often occurs in proximal colon cancers, and molecular phenotypes underlying MSI cancers in distal colon have not been fully clarified. Our goal was to clarify the difference between MSI and microsatellite stability (MSS) cancers and, furthermore, to determine distinct characteristics of proximal and distal MSI cancers. By DNA microarray analysis of 84 cancers (33 MSI and 51 MSS), we identified discriminating genes (177 probe sets), which predicted MSI status with a high accuracy rate (97.6%). These genes were related to phenotypic characteristics of MSI cancers. Next, we identified 24 probe sets that were differentially expressed in proximal and distal MSI cancers. These genes included promoter methylation-mediated genes, whose expression was significantly down-regulated in proximal MSI cancers. Among discriminating genes between MSI and MSS, nine methylation-mediated genes showed down-regulation in MSI cancers. Of these, 7 (77.8%) showed down-regulation in proximal MSI cancers. Furthermore, methylation-specific PCR confirmed that frequency of hMLH1 promoter methylation was significantly higher in proximal MSI cancers (P = 0.0317). These results suggested that there is a difference between proximal and distal MSI cancers in methylation-mediated influence on gene silencing. In conclusion, using DNA microarray, we could distinguish MSI and MSS cancers. We also showed distinct characteristics of proximal and distal MSI cancers. The inactivation form of hMLH, per se, differed between proximal and distal MSI cancers. These results suggested that distal MSI cancers constitute a distinct subgroup of sporadic MSI cancers.

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