Distance-Based Tear Lactoferrin Assay on Microfluidic Paper Device Using Interfacial Interactions on Surface-Modified Cellulose

Kentaro Yamada, Terence G. Henares, Koji Suzuki, Daniel Citterio

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Distance-based detection motifs on microfluidic paper-based analytical devices (μPADs) allow quantitative analysis without using signal readout instruments in a similar manner to classical analogue thermometers. To realize a cost-effective and calibration-free distance-based assay of lactoferrin in human tear fluid on a μPAD not relying on antibodies or enzymes, we investigated the fluidic mobilities of the target protein and Tb3+ cations used as the fluorescent detection reagent on surface-modified cellulosic filter papers. Chromatographic elution experiments in a tear-like sample matrix containing electrolytes and proteins revealed a collapse of attractive electrostatic interactions between lactoferrin or Tb3+ and the cellulosic substrate, which was overcome by the modification of the paper surface with the sulfated polysaccharide I-carrageenan. The resulting μPAD based on the fluorescence emission distance successfully analyzed 0-4 mg mL-1 of lactoferrin in complex human tear matrix with a lower limit of detection of 0.1 mg mL-1 by simple visual inspection. Assay results of 18 human tear samples including ocular disease patients and healthy volunteers showed good correlation to the reference ELISA method with a slope of 0.997 and a regression coefficient of 0.948. The distance-based quantitative signal and the good batch-to-batch fabrication reproducibility relying on printing methods enable quantitative analysis by simply reading out concentration scale marks printed on the μPAD without performing any calibration and using any signal readout instrument.

Original languageEnglish
Pages (from-to)24864-24875
Number of pages12
JournalACS Applied Materials and Interfaces
Volume7
Issue number44
DOIs
Publication statusPublished - 2015 Nov 11

Fingerprint

Readout systems
Lactoferrin
Microfluidics
Cellulose
Assays
Calibration
Proteins
Thermometers
Fluidics
Polysaccharides
Coulomb interactions
Chemical analysis
Antibodies
Printing
Enzymes
Carrageenan
Inspection
Positive ions
Fluorescence
Electrolytes

Keywords

  • anionic polysaccharide
  • cation immobilization
  • paper-based analytical device
  • protein mobility
  • surface modification

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Distance-Based Tear Lactoferrin Assay on Microfluidic Paper Device Using Interfacial Interactions on Surface-Modified Cellulose. / Yamada, Kentaro; Henares, Terence G.; Suzuki, Koji; Citterio, Daniel.

In: ACS Applied Materials and Interfaces, Vol. 7, No. 44, 11.11.2015, p. 24864-24875.

Research output: Contribution to journalArticle

@article{2f64f3516e644bfc9cc901601e5db0be,
title = "Distance-Based Tear Lactoferrin Assay on Microfluidic Paper Device Using Interfacial Interactions on Surface-Modified Cellulose",
abstract = "Distance-based detection motifs on microfluidic paper-based analytical devices (μPADs) allow quantitative analysis without using signal readout instruments in a similar manner to classical analogue thermometers. To realize a cost-effective and calibration-free distance-based assay of lactoferrin in human tear fluid on a μPAD not relying on antibodies or enzymes, we investigated the fluidic mobilities of the target protein and Tb3+ cations used as the fluorescent detection reagent on surface-modified cellulosic filter papers. Chromatographic elution experiments in a tear-like sample matrix containing electrolytes and proteins revealed a collapse of attractive electrostatic interactions between lactoferrin or Tb3+ and the cellulosic substrate, which was overcome by the modification of the paper surface with the sulfated polysaccharide I-carrageenan. The resulting μPAD based on the fluorescence emission distance successfully analyzed 0-4 mg mL-1 of lactoferrin in complex human tear matrix with a lower limit of detection of 0.1 mg mL-1 by simple visual inspection. Assay results of 18 human tear samples including ocular disease patients and healthy volunteers showed good correlation to the reference ELISA method with a slope of 0.997 and a regression coefficient of 0.948. The distance-based quantitative signal and the good batch-to-batch fabrication reproducibility relying on printing methods enable quantitative analysis by simply reading out concentration scale marks printed on the μPAD without performing any calibration and using any signal readout instrument.",
keywords = "anionic polysaccharide, cation immobilization, paper-based analytical device, protein mobility, surface modification",
author = "Kentaro Yamada and Henares, {Terence G.} and Koji Suzuki and Daniel Citterio",
year = "2015",
month = "11",
day = "11",
doi = "10.1021/acsami.5b08124",
language = "English",
volume = "7",
pages = "24864--24875",
journal = "ACS applied materials & interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "44",

}

TY - JOUR

T1 - Distance-Based Tear Lactoferrin Assay on Microfluidic Paper Device Using Interfacial Interactions on Surface-Modified Cellulose

AU - Yamada, Kentaro

AU - Henares, Terence G.

AU - Suzuki, Koji

AU - Citterio, Daniel

PY - 2015/11/11

Y1 - 2015/11/11

N2 - Distance-based detection motifs on microfluidic paper-based analytical devices (μPADs) allow quantitative analysis without using signal readout instruments in a similar manner to classical analogue thermometers. To realize a cost-effective and calibration-free distance-based assay of lactoferrin in human tear fluid on a μPAD not relying on antibodies or enzymes, we investigated the fluidic mobilities of the target protein and Tb3+ cations used as the fluorescent detection reagent on surface-modified cellulosic filter papers. Chromatographic elution experiments in a tear-like sample matrix containing electrolytes and proteins revealed a collapse of attractive electrostatic interactions between lactoferrin or Tb3+ and the cellulosic substrate, which was overcome by the modification of the paper surface with the sulfated polysaccharide I-carrageenan. The resulting μPAD based on the fluorescence emission distance successfully analyzed 0-4 mg mL-1 of lactoferrin in complex human tear matrix with a lower limit of detection of 0.1 mg mL-1 by simple visual inspection. Assay results of 18 human tear samples including ocular disease patients and healthy volunteers showed good correlation to the reference ELISA method with a slope of 0.997 and a regression coefficient of 0.948. The distance-based quantitative signal and the good batch-to-batch fabrication reproducibility relying on printing methods enable quantitative analysis by simply reading out concentration scale marks printed on the μPAD without performing any calibration and using any signal readout instrument.

AB - Distance-based detection motifs on microfluidic paper-based analytical devices (μPADs) allow quantitative analysis without using signal readout instruments in a similar manner to classical analogue thermometers. To realize a cost-effective and calibration-free distance-based assay of lactoferrin in human tear fluid on a μPAD not relying on antibodies or enzymes, we investigated the fluidic mobilities of the target protein and Tb3+ cations used as the fluorescent detection reagent on surface-modified cellulosic filter papers. Chromatographic elution experiments in a tear-like sample matrix containing electrolytes and proteins revealed a collapse of attractive electrostatic interactions between lactoferrin or Tb3+ and the cellulosic substrate, which was overcome by the modification of the paper surface with the sulfated polysaccharide I-carrageenan. The resulting μPAD based on the fluorescence emission distance successfully analyzed 0-4 mg mL-1 of lactoferrin in complex human tear matrix with a lower limit of detection of 0.1 mg mL-1 by simple visual inspection. Assay results of 18 human tear samples including ocular disease patients and healthy volunteers showed good correlation to the reference ELISA method with a slope of 0.997 and a regression coefficient of 0.948. The distance-based quantitative signal and the good batch-to-batch fabrication reproducibility relying on printing methods enable quantitative analysis by simply reading out concentration scale marks printed on the μPAD without performing any calibration and using any signal readout instrument.

KW - anionic polysaccharide

KW - cation immobilization

KW - paper-based analytical device

KW - protein mobility

KW - surface modification

UR - http://www.scopus.com/inward/record.url?scp=84947080936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947080936&partnerID=8YFLogxK

U2 - 10.1021/acsami.5b08124

DO - 10.1021/acsami.5b08124

M3 - Article

C2 - 26488371

AN - SCOPUS:84947080936

VL - 7

SP - 24864

EP - 24875

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

SN - 1944-8244

IS - 44

ER -