Distinct and Collaborative Functions of Yb and Armitage in Transposon-Targeting piRNA Biogenesis

Hirotsugu Ishizu, Tatsuki Kinoshita, Shigeki Hirakata, Chihiro Komatsuzaki, Mikiko C. Siomi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

PIWI-interacting RNAs (piRNAs) repress transposons to maintain germline genome integrity. Previous studies showed that artificial tethering of Armitage (Armi) to reporter RNAs induced piRNA biogenesis. However, the lack of female sterile (1) Yb (Yb) in Drosophila ovarian somatic cells (OSCs) impaired the production of transposon-targeting piRNAs, even in the presence of Armi. Here, we show that the specific interaction of Armi with RNA transcripts of the flamenco piRNA cluster, the primary source of transposon-targeting piRNAs in OSCs, is strictly regulated by Yb. The lack of Yb allowed Armi to bind RNAs promiscuously, leading to the production of piRNAs unrelated to transposon silencing. The ATP hydrolysis-defective mutants of Armi failed to unwind RNAs and were retained on them, abolishing piRNA production. These findings shed light on distinct and collaborative requirements of Yb and Armi in transposon-targeting piRNA biogenesis. We also provide evidence supporting the direct involvement of Armi but not Yb in Zucchini-dependent piRNA phasing. Ishizu et al. show that the selective binding of Armi to piRNA precursors is controlled by Yb and the ATP hydrolysis activity of Armi in Drosophila ovarian somatic cells. The data presented here also suggest that Armi is directly involved in Zuc-dependent phased piRNAs biogenesis.

Original languageEnglish
Pages (from-to)1822-1835.e8
JournalCell Reports
Volume27
Issue number6
DOIs
Publication statusPublished - 2019 May 7
Externally publishedYes

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Small Interfering RNA
RNA
Drosophila
Hydrolysis
Adenosine Triphosphate
Genes
Genome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Distinct and Collaborative Functions of Yb and Armitage in Transposon-Targeting piRNA Biogenesis. / Ishizu, Hirotsugu; Kinoshita, Tatsuki; Hirakata, Shigeki; Komatsuzaki, Chihiro; Siomi, Mikiko C.

In: Cell Reports, Vol. 27, No. 6, 07.05.2019, p. 1822-1835.e8.

Research output: Contribution to journalArticle

Ishizu, Hirotsugu ; Kinoshita, Tatsuki ; Hirakata, Shigeki ; Komatsuzaki, Chihiro ; Siomi, Mikiko C. / Distinct and Collaborative Functions of Yb and Armitage in Transposon-Targeting piRNA Biogenesis. In: Cell Reports. 2019 ; Vol. 27, No. 6. pp. 1822-1835.e8.
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