Distinct expression of Cbln family mRNAs in developing and adult mouse brains

Eriko Miura, Takatoshi Iijima, Michisuke Yuzaki, Masahiko Watanabe

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Cbln1 belongs to the C1q and tumour necrosis factor superfamily, and plays crucial roles as a cerebellar granule cell-derived transneuronal regulator for synapse integrity and plasticity in Purkinje cells. Although Cbln2-Cbln4 are also expressed in the brain and could form heteromeric complexes with Cbln1, their precise expressions remain unclear. Here, we investigated gene expression of the Cbln family in developing and adult C57BL mouse brains by reverse transcriptase-polymerase chain reaction (RT-PCR), Northern blot, and high-resolution in situ hybridization (ISH) analyses. In the adult brain, spatial patterns of mRNA expression were highly differential depending on Cbln subtypes. Notably, particularly high levels of Cbln mRNAs were expressed in some nuclei and neurons, whereas their postsynaptic targets often lacked or were low for any Cbln mRNAs, as seen for cerebellar granule cells/Purkinje cells, entorhinal cortex/hippocampus, intralaminar group of thalamic nuclei/caudate-putamen, and dorsal nucleus of the lateral lemniscus/central nucleus of the inferior colliculus. In the developing brain, Cbln1, 2, and 4 mRNAs appeared as early as embryonic day 10-13, and exhibited transient up-regulation during the late embryonic and neonatal periods. For example, Cbln2 mRNA was expressed in the cortical plate of the developing neocortex, displaying a high rostromedial to low caudolateral gradient. In contrast, Cbln3 mRNA was selective to cerebellar granule cells throughout development, and its onset was as late as postnatal day 7-10. These results will provide a molecular-anatomical basis for future studies that characterize roles played by the Cbln family.

Original languageEnglish
Pages (from-to)750-760
Number of pages11
JournalEuropean Journal of Neuroscience
Volume24
Issue number3
DOIs
Publication statusPublished - 2006 Aug

Fingerprint

Messenger RNA
Brain
Purkinje Cells
Intralaminar Thalamic Nuclei
Inferior Colliculi
Entorhinal Cortex
Putamen
Neocortex
Reverse Transcriptase Polymerase Chain Reaction
Inbred C57BL Mouse
Northern Blotting
Cerebral Cortex
Synapses
In Situ Hybridization
Hippocampus
Up-Regulation
Tumor Necrosis Factor-alpha
Gene Expression
Neurons

Keywords

  • C57BL mice
  • Cerebellin
  • in situ hybridization
  • Northern blot
  • RT-PCR
  • Synapse development

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distinct expression of Cbln family mRNAs in developing and adult mouse brains. / Miura, Eriko; Iijima, Takatoshi; Yuzaki, Michisuke; Watanabe, Masahiko.

In: European Journal of Neuroscience, Vol. 24, No. 3, 08.2006, p. 750-760.

Research output: Contribution to journalArticle

Miura, Eriko ; Iijima, Takatoshi ; Yuzaki, Michisuke ; Watanabe, Masahiko. / Distinct expression of Cbln family mRNAs in developing and adult mouse brains. In: European Journal of Neuroscience. 2006 ; Vol. 24, No. 3. pp. 750-760.
@article{e84df047bd5d488cb4c7f8c8d2a3eb56,
title = "Distinct expression of Cbln family mRNAs in developing and adult mouse brains",
abstract = "Cbln1 belongs to the C1q and tumour necrosis factor superfamily, and plays crucial roles as a cerebellar granule cell-derived transneuronal regulator for synapse integrity and plasticity in Purkinje cells. Although Cbln2-Cbln4 are also expressed in the brain and could form heteromeric complexes with Cbln1, their precise expressions remain unclear. Here, we investigated gene expression of the Cbln family in developing and adult C57BL mouse brains by reverse transcriptase-polymerase chain reaction (RT-PCR), Northern blot, and high-resolution in situ hybridization (ISH) analyses. In the adult brain, spatial patterns of mRNA expression were highly differential depending on Cbln subtypes. Notably, particularly high levels of Cbln mRNAs were expressed in some nuclei and neurons, whereas their postsynaptic targets often lacked or were low for any Cbln mRNAs, as seen for cerebellar granule cells/Purkinje cells, entorhinal cortex/hippocampus, intralaminar group of thalamic nuclei/caudate-putamen, and dorsal nucleus of the lateral lemniscus/central nucleus of the inferior colliculus. In the developing brain, Cbln1, 2, and 4 mRNAs appeared as early as embryonic day 10-13, and exhibited transient up-regulation during the late embryonic and neonatal periods. For example, Cbln2 mRNA was expressed in the cortical plate of the developing neocortex, displaying a high rostromedial to low caudolateral gradient. In contrast, Cbln3 mRNA was selective to cerebellar granule cells throughout development, and its onset was as late as postnatal day 7-10. These results will provide a molecular-anatomical basis for future studies that characterize roles played by the Cbln family.",
keywords = "C57BL mice, Cerebellin, in situ hybridization, Northern blot, RT-PCR, Synapse development",
author = "Eriko Miura and Takatoshi Iijima and Michisuke Yuzaki and Masahiko Watanabe",
year = "2006",
month = "8",
doi = "10.1111/j.1460-9568.2006.04950.x",
language = "English",
volume = "24",
pages = "750--760",
journal = "European Journal of Neuroscience",
issn = "0953-816X",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Distinct expression of Cbln family mRNAs in developing and adult mouse brains

AU - Miura, Eriko

AU - Iijima, Takatoshi

AU - Yuzaki, Michisuke

AU - Watanabe, Masahiko

PY - 2006/8

Y1 - 2006/8

N2 - Cbln1 belongs to the C1q and tumour necrosis factor superfamily, and plays crucial roles as a cerebellar granule cell-derived transneuronal regulator for synapse integrity and plasticity in Purkinje cells. Although Cbln2-Cbln4 are also expressed in the brain and could form heteromeric complexes with Cbln1, their precise expressions remain unclear. Here, we investigated gene expression of the Cbln family in developing and adult C57BL mouse brains by reverse transcriptase-polymerase chain reaction (RT-PCR), Northern blot, and high-resolution in situ hybridization (ISH) analyses. In the adult brain, spatial patterns of mRNA expression were highly differential depending on Cbln subtypes. Notably, particularly high levels of Cbln mRNAs were expressed in some nuclei and neurons, whereas their postsynaptic targets often lacked or were low for any Cbln mRNAs, as seen for cerebellar granule cells/Purkinje cells, entorhinal cortex/hippocampus, intralaminar group of thalamic nuclei/caudate-putamen, and dorsal nucleus of the lateral lemniscus/central nucleus of the inferior colliculus. In the developing brain, Cbln1, 2, and 4 mRNAs appeared as early as embryonic day 10-13, and exhibited transient up-regulation during the late embryonic and neonatal periods. For example, Cbln2 mRNA was expressed in the cortical plate of the developing neocortex, displaying a high rostromedial to low caudolateral gradient. In contrast, Cbln3 mRNA was selective to cerebellar granule cells throughout development, and its onset was as late as postnatal day 7-10. These results will provide a molecular-anatomical basis for future studies that characterize roles played by the Cbln family.

AB - Cbln1 belongs to the C1q and tumour necrosis factor superfamily, and plays crucial roles as a cerebellar granule cell-derived transneuronal regulator for synapse integrity and plasticity in Purkinje cells. Although Cbln2-Cbln4 are also expressed in the brain and could form heteromeric complexes with Cbln1, their precise expressions remain unclear. Here, we investigated gene expression of the Cbln family in developing and adult C57BL mouse brains by reverse transcriptase-polymerase chain reaction (RT-PCR), Northern blot, and high-resolution in situ hybridization (ISH) analyses. In the adult brain, spatial patterns of mRNA expression were highly differential depending on Cbln subtypes. Notably, particularly high levels of Cbln mRNAs were expressed in some nuclei and neurons, whereas their postsynaptic targets often lacked or were low for any Cbln mRNAs, as seen for cerebellar granule cells/Purkinje cells, entorhinal cortex/hippocampus, intralaminar group of thalamic nuclei/caudate-putamen, and dorsal nucleus of the lateral lemniscus/central nucleus of the inferior colliculus. In the developing brain, Cbln1, 2, and 4 mRNAs appeared as early as embryonic day 10-13, and exhibited transient up-regulation during the late embryonic and neonatal periods. For example, Cbln2 mRNA was expressed in the cortical plate of the developing neocortex, displaying a high rostromedial to low caudolateral gradient. In contrast, Cbln3 mRNA was selective to cerebellar granule cells throughout development, and its onset was as late as postnatal day 7-10. These results will provide a molecular-anatomical basis for future studies that characterize roles played by the Cbln family.

KW - C57BL mice

KW - Cerebellin

KW - in situ hybridization

KW - Northern blot

KW - RT-PCR

KW - Synapse development

UR - http://www.scopus.com/inward/record.url?scp=33747175917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747175917&partnerID=8YFLogxK

U2 - 10.1111/j.1460-9568.2006.04950.x

DO - 10.1111/j.1460-9568.2006.04950.x

M3 - Article

C2 - 16930405

AN - SCOPUS:33747175917

VL - 24

SP - 750

EP - 760

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

SN - 0953-816X

IS - 3

ER -