Distinct gene expression profiles characterize cellular phenotypes of follicle-associated epithelium and M cells

Kouji Hase, Sayaka Ohshima, Kazuya Kawano, Noriko Hashimoto, Kenji Matsumoto, Hirohisa Saito, Hiroshi Ohno

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Follicle-associated epithelium (FAE) covering Peyer's patches contains specialized epithelial M cells that take up ingested macromolecules and microorganisms from the lumen of the gut by transcytosis. Using high-density oligonucleotide microarrays, we analyzed the gene expression profiles of FAE and M cells in order to characterize their cellular phenotypes. The microarray data revealed that, among approximately 14,000 genes, 409 were expressed in FAE at twofold or higher levels compared to the intestinal epithelial cells (IECs) of the villi. These included genes involved in membrane traffic, host defense and transcriptional regulation, as well as uncharacterized genes. Real-time PCR and in situ hybridization analyses identified three molecules, ubiquitin D (Ub-D), tumor necrosis factor receptor superfamily 12a (TNFRsf12a), and transmembrane 4 superfamily 4 (Tm4sf4), which were predominantly distributed throughout FAE, but were expressed little, if at all, in IECs. By contrast, transcripts of secretory granule neuroendocrine protein 1 (Sgne-1) were scattered in FAE, and were co-localized with Ulex europaeus agglutinin-1 (UEA-1)-positive cells. This clearly suggests that expression of Sgne-1 in the gut is specific to M cells. Such a unique pattern of gene expression distinguishes FAE and M cells from IECs, and may reflect their cellular phenotype(s) associated with specific functional features.

Original languageEnglish
Pages (from-to)127-137
Number of pages11
JournalDNA Research
Volume12
Issue number2
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Transcriptome
Epithelium
Phenotype
Epithelial Cells
Secretory Vesicles
Genes
Transcytosis
Peyer's Patches
Tumor Necrosis Factor Receptors
Ubiquitin
Oligonucleotide Array Sequence Analysis
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Proteins
Gene Expression
Membranes

Keywords

  • Affymetrix
  • FAE
  • M cells
  • Microarray
  • Sgne-1

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Distinct gene expression profiles characterize cellular phenotypes of follicle-associated epithelium and M cells. / Hase, Kouji; Ohshima, Sayaka; Kawano, Kazuya; Hashimoto, Noriko; Matsumoto, Kenji; Saito, Hirohisa; Ohno, Hiroshi.

In: DNA Research, Vol. 12, No. 2, 2005, p. 127-137.

Research output: Contribution to journalArticle

Hase, Kouji ; Ohshima, Sayaka ; Kawano, Kazuya ; Hashimoto, Noriko ; Matsumoto, Kenji ; Saito, Hirohisa ; Ohno, Hiroshi. / Distinct gene expression profiles characterize cellular phenotypes of follicle-associated epithelium and M cells. In: DNA Research. 2005 ; Vol. 12, No. 2. pp. 127-137.
@article{06b3bdbc275e41d7b1c58ee45b1c6bd2,
title = "Distinct gene expression profiles characterize cellular phenotypes of follicle-associated epithelium and M cells",
abstract = "Follicle-associated epithelium (FAE) covering Peyer's patches contains specialized epithelial M cells that take up ingested macromolecules and microorganisms from the lumen of the gut by transcytosis. Using high-density oligonucleotide microarrays, we analyzed the gene expression profiles of FAE and M cells in order to characterize their cellular phenotypes. The microarray data revealed that, among approximately 14,000 genes, 409 were expressed in FAE at twofold or higher levels compared to the intestinal epithelial cells (IECs) of the villi. These included genes involved in membrane traffic, host defense and transcriptional regulation, as well as uncharacterized genes. Real-time PCR and in situ hybridization analyses identified three molecules, ubiquitin D (Ub-D), tumor necrosis factor receptor superfamily 12a (TNFRsf12a), and transmembrane 4 superfamily 4 (Tm4sf4), which were predominantly distributed throughout FAE, but were expressed little, if at all, in IECs. By contrast, transcripts of secretory granule neuroendocrine protein 1 (Sgne-1) were scattered in FAE, and were co-localized with Ulex europaeus agglutinin-1 (UEA-1)-positive cells. This clearly suggests that expression of Sgne-1 in the gut is specific to M cells. Such a unique pattern of gene expression distinguishes FAE and M cells from IECs, and may reflect their cellular phenotype(s) associated with specific functional features.",
keywords = "Affymetrix, FAE, M cells, Microarray, Sgne-1",
author = "Kouji Hase and Sayaka Ohshima and Kazuya Kawano and Noriko Hashimoto and Kenji Matsumoto and Hirohisa Saito and Hiroshi Ohno",
year = "2005",
doi = "10.1093/dnares/12.2.127",
language = "English",
volume = "12",
pages = "127--137",
journal = "DNA Research",
issn = "1340-2838",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Distinct gene expression profiles characterize cellular phenotypes of follicle-associated epithelium and M cells

AU - Hase, Kouji

AU - Ohshima, Sayaka

AU - Kawano, Kazuya

AU - Hashimoto, Noriko

AU - Matsumoto, Kenji

AU - Saito, Hirohisa

AU - Ohno, Hiroshi

PY - 2005

Y1 - 2005

N2 - Follicle-associated epithelium (FAE) covering Peyer's patches contains specialized epithelial M cells that take up ingested macromolecules and microorganisms from the lumen of the gut by transcytosis. Using high-density oligonucleotide microarrays, we analyzed the gene expression profiles of FAE and M cells in order to characterize their cellular phenotypes. The microarray data revealed that, among approximately 14,000 genes, 409 were expressed in FAE at twofold or higher levels compared to the intestinal epithelial cells (IECs) of the villi. These included genes involved in membrane traffic, host defense and transcriptional regulation, as well as uncharacterized genes. Real-time PCR and in situ hybridization analyses identified three molecules, ubiquitin D (Ub-D), tumor necrosis factor receptor superfamily 12a (TNFRsf12a), and transmembrane 4 superfamily 4 (Tm4sf4), which were predominantly distributed throughout FAE, but were expressed little, if at all, in IECs. By contrast, transcripts of secretory granule neuroendocrine protein 1 (Sgne-1) were scattered in FAE, and were co-localized with Ulex europaeus agglutinin-1 (UEA-1)-positive cells. This clearly suggests that expression of Sgne-1 in the gut is specific to M cells. Such a unique pattern of gene expression distinguishes FAE and M cells from IECs, and may reflect their cellular phenotype(s) associated with specific functional features.

AB - Follicle-associated epithelium (FAE) covering Peyer's patches contains specialized epithelial M cells that take up ingested macromolecules and microorganisms from the lumen of the gut by transcytosis. Using high-density oligonucleotide microarrays, we analyzed the gene expression profiles of FAE and M cells in order to characterize their cellular phenotypes. The microarray data revealed that, among approximately 14,000 genes, 409 were expressed in FAE at twofold or higher levels compared to the intestinal epithelial cells (IECs) of the villi. These included genes involved in membrane traffic, host defense and transcriptional regulation, as well as uncharacterized genes. Real-time PCR and in situ hybridization analyses identified three molecules, ubiquitin D (Ub-D), tumor necrosis factor receptor superfamily 12a (TNFRsf12a), and transmembrane 4 superfamily 4 (Tm4sf4), which were predominantly distributed throughout FAE, but were expressed little, if at all, in IECs. By contrast, transcripts of secretory granule neuroendocrine protein 1 (Sgne-1) were scattered in FAE, and were co-localized with Ulex europaeus agglutinin-1 (UEA-1)-positive cells. This clearly suggests that expression of Sgne-1 in the gut is specific to M cells. Such a unique pattern of gene expression distinguishes FAE and M cells from IECs, and may reflect their cellular phenotype(s) associated with specific functional features.

KW - Affymetrix

KW - FAE

KW - M cells

KW - Microarray

KW - Sgne-1

UR - http://www.scopus.com/inward/record.url?scp=24944576523&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24944576523&partnerID=8YFLogxK

U2 - 10.1093/dnares/12.2.127

DO - 10.1093/dnares/12.2.127

M3 - Article

VL - 12

SP - 127

EP - 137

JO - DNA Research

JF - DNA Research

SN - 1340-2838

IS - 2

ER -