Distinct modulation of superficial and juxtamedullary arterioles by prostaglandin in vivo

Hiroto Matsuda, Koichi Hayashi, Koki Arakawa, Eiji Kubota, Masanori Honda, Hirobumi Tokuyama, Hiromichi Suzuki, Tokunori Yamamoto, Fumihiko Kajiya, Takao Saruta

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Renal afferent (AFF) and efferent arteriolar (EFF) responsiveness to angiotensin II (ANG II) in superficial and juxtamedullary nephrons in vivo remains undetermined, nor has it been clarified what role intrarenal autocrines/paracrines play in modulating the renal microvascular response. The present study characterized the responsiveness to ANG II (1-30 ng/kg/min) of AFF and EFF of canine superficial and juxtamedullary nephrons under pentobarbital anesthesia, using intravital CCD-videomicroscopy that allowed direct in vivo visualization of the renal microcirculation. Furthermore, the effect of prostaglandins (PG) and nitric oxide (NO) on ANG II-induced tone was examined. In superficial nephrons, ANG II induced a similar dose-dependent constriction of both AFF (46±5% constriction) and EFF (53±3%). In juxtamedullary arterioles, ANG II induced a dose-dependent constriction of EFF, whereas AFF responses were diminished (17±4% vs. 37±4% at 10 ng/kg/min). The PG inhibition by indomethacin enhanced the ANG II-induced constriction of juxtamedullary AFF, whereas no augmentation was observed in other arterioles. In contrast, NO inhibition by nitro-L-arginine methylester (L-NAME) enhanced the ANG II-induced constriction, with greater augmentation in juxtamedullary AFF and EFF. Finally, renal interstitial PG and nitrite/nitrate contents were greater in the medulla than the superficial cortex under basal and ANG II-stimulated conditions. Taken together, the results of the intravital CCD-videomicroscopy reveal that the renal microvascular action of ANG II had both zonal (juxtamedullary vs. superficial nephrons) and segmental (AFF vs. EFF) heterogeneity under the present experimental conditions. This heterogeneity was associated with a difference in the intrarenal production of prostaglandin E2 (PGE2) and NO; PGE2 contributed to segmental and zonal differences whereas NO was responsible for the zonal heterogeneity in arteriolar responsiveness.

Original languageEnglish
Pages (from-to)901-910
Number of pages10
JournalHypertension Research
Volume25
Issue number6
Publication statusPublished - 2002 Nov

Fingerprint

Arterioles
Angiotensin II
Prostaglandins
Constriction
Nephrons
Kidney
Nitric Oxide
Video Microscopy
Dinoprostone
NG-Nitroarginine Methyl Ester
Pentobarbital
Microcirculation
Nitrites
Indomethacin
Nitrates
Arginine
Canidae
Anesthesia

Keywords

  • Afferent arterioles
  • Angiotensin II
  • Efferent arterioles
  • Nitric oxide
  • Prostaglandin

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Matsuda, H., Hayashi, K., Arakawa, K., Kubota, E., Honda, M., Tokuyama, H., ... Saruta, T. (2002). Distinct modulation of superficial and juxtamedullary arterioles by prostaglandin in vivo. Hypertension Research, 25(6), 901-910.

Distinct modulation of superficial and juxtamedullary arterioles by prostaglandin in vivo. / Matsuda, Hiroto; Hayashi, Koichi; Arakawa, Koki; Kubota, Eiji; Honda, Masanori; Tokuyama, Hirobumi; Suzuki, Hiromichi; Yamamoto, Tokunori; Kajiya, Fumihiko; Saruta, Takao.

In: Hypertension Research, Vol. 25, No. 6, 11.2002, p. 901-910.

Research output: Contribution to journalArticle

Matsuda, H, Hayashi, K, Arakawa, K, Kubota, E, Honda, M, Tokuyama, H, Suzuki, H, Yamamoto, T, Kajiya, F & Saruta, T 2002, 'Distinct modulation of superficial and juxtamedullary arterioles by prostaglandin in vivo', Hypertension Research, vol. 25, no. 6, pp. 901-910.
Matsuda H, Hayashi K, Arakawa K, Kubota E, Honda M, Tokuyama H et al. Distinct modulation of superficial and juxtamedullary arterioles by prostaglandin in vivo. Hypertension Research. 2002 Nov;25(6):901-910.
Matsuda, Hiroto ; Hayashi, Koichi ; Arakawa, Koki ; Kubota, Eiji ; Honda, Masanori ; Tokuyama, Hirobumi ; Suzuki, Hiromichi ; Yamamoto, Tokunori ; Kajiya, Fumihiko ; Saruta, Takao. / Distinct modulation of superficial and juxtamedullary arterioles by prostaglandin in vivo. In: Hypertension Research. 2002 ; Vol. 25, No. 6. pp. 901-910.
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abstract = "Renal afferent (AFF) and efferent arteriolar (EFF) responsiveness to angiotensin II (ANG II) in superficial and juxtamedullary nephrons in vivo remains undetermined, nor has it been clarified what role intrarenal autocrines/paracrines play in modulating the renal microvascular response. The present study characterized the responsiveness to ANG II (1-30 ng/kg/min) of AFF and EFF of canine superficial and juxtamedullary nephrons under pentobarbital anesthesia, using intravital CCD-videomicroscopy that allowed direct in vivo visualization of the renal microcirculation. Furthermore, the effect of prostaglandins (PG) and nitric oxide (NO) on ANG II-induced tone was examined. In superficial nephrons, ANG II induced a similar dose-dependent constriction of both AFF (46±5{\%} constriction) and EFF (53±3{\%}). In juxtamedullary arterioles, ANG II induced a dose-dependent constriction of EFF, whereas AFF responses were diminished (17±4{\%} vs. 37±4{\%} at 10 ng/kg/min). The PG inhibition by indomethacin enhanced the ANG II-induced constriction of juxtamedullary AFF, whereas no augmentation was observed in other arterioles. In contrast, NO inhibition by nitro-L-arginine methylester (L-NAME) enhanced the ANG II-induced constriction, with greater augmentation in juxtamedullary AFF and EFF. Finally, renal interstitial PG and nitrite/nitrate contents were greater in the medulla than the superficial cortex under basal and ANG II-stimulated conditions. Taken together, the results of the intravital CCD-videomicroscopy reveal that the renal microvascular action of ANG II had both zonal (juxtamedullary vs. superficial nephrons) and segmental (AFF vs. EFF) heterogeneity under the present experimental conditions. This heterogeneity was associated with a difference in the intrarenal production of prostaglandin E2 (PGE2) and NO; PGE2 contributed to segmental and zonal differences whereas NO was responsible for the zonal heterogeneity in arteriolar responsiveness.",
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AU - Hayashi, Koichi

AU - Arakawa, Koki

AU - Kubota, Eiji

AU - Honda, Masanori

AU - Tokuyama, Hirobumi

AU - Suzuki, Hiromichi

AU - Yamamoto, Tokunori

AU - Kajiya, Fumihiko

AU - Saruta, Takao

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N2 - Renal afferent (AFF) and efferent arteriolar (EFF) responsiveness to angiotensin II (ANG II) in superficial and juxtamedullary nephrons in vivo remains undetermined, nor has it been clarified what role intrarenal autocrines/paracrines play in modulating the renal microvascular response. The present study characterized the responsiveness to ANG II (1-30 ng/kg/min) of AFF and EFF of canine superficial and juxtamedullary nephrons under pentobarbital anesthesia, using intravital CCD-videomicroscopy that allowed direct in vivo visualization of the renal microcirculation. Furthermore, the effect of prostaglandins (PG) and nitric oxide (NO) on ANG II-induced tone was examined. In superficial nephrons, ANG II induced a similar dose-dependent constriction of both AFF (46±5% constriction) and EFF (53±3%). In juxtamedullary arterioles, ANG II induced a dose-dependent constriction of EFF, whereas AFF responses were diminished (17±4% vs. 37±4% at 10 ng/kg/min). The PG inhibition by indomethacin enhanced the ANG II-induced constriction of juxtamedullary AFF, whereas no augmentation was observed in other arterioles. In contrast, NO inhibition by nitro-L-arginine methylester (L-NAME) enhanced the ANG II-induced constriction, with greater augmentation in juxtamedullary AFF and EFF. Finally, renal interstitial PG and nitrite/nitrate contents were greater in the medulla than the superficial cortex under basal and ANG II-stimulated conditions. Taken together, the results of the intravital CCD-videomicroscopy reveal that the renal microvascular action of ANG II had both zonal (juxtamedullary vs. superficial nephrons) and segmental (AFF vs. EFF) heterogeneity under the present experimental conditions. This heterogeneity was associated with a difference in the intrarenal production of prostaglandin E2 (PGE2) and NO; PGE2 contributed to segmental and zonal differences whereas NO was responsible for the zonal heterogeneity in arteriolar responsiveness.

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