Distinct Roles for the N-and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation

Shunsuke Kimura, Masami Yamashita, Megumi Yamakami-Kimura, Yusuke Sato, Atsushi Yamagata, Yoshihiro Kobashigawa, Fuyuhiko Inagaki, Takako Amada, Koji Hase, Toshihiko Iwanaga, Hiroshi Ohno, Shuya Fukai

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15 Citations (Scopus)

Abstract

The tunneling nanotube (TNT) is a structure used for intercellular communication, and is a thin membrane protrusion mediating transport of various signaling molecules and cellular components. M-Sec has potent membrane deformation ability and induces TNT formation in cooperation with the Ral/exocyst complex. Here, we show that the N-terminal polybasic region of M-Sec directly binds phosphatidylinositol (4,5)-bisphosphate for its localization to the plasma membrane during the initial stage of TNT formation. We further report a crystal structure of M-Sec, which consists of helix bundles arranged in a straight rod-like shape, similar to the membrane tethering complex subunits. A positively charged surface in the C-terminal domains is required for M-Sec interaction with active RalA to extend the plasma membrane protrusions. Our results suggest that the membrane-associated M-Sec recruits active RalA, which directs the exocyst complex to form TNTs.

Original languageEnglish
Article number33548
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Sep 15

ASJC Scopus subject areas

  • General

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    Kimura, S., Yamashita, M., Yamakami-Kimura, M., Sato, Y., Yamagata, A., Kobashigawa, Y., Inagaki, F., Amada, T., Hase, K., Iwanaga, T., Ohno, H., & Fukai, S. (2016). Distinct Roles for the N-and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation. Scientific reports, 6, [33548]. https://doi.org/10.1038/srep33548