Distinct Roles for the N-and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation

Shunsuke Kimura, Masami Yamashita, Megumi Yamakami-Kimura, Yusuke Sato, Atsushi Yamagata, Yoshihiro Kobashigawa, Fuyuhiko Inagaki, Takako Amada, Kouji Hase, Toshihiko Iwanaga, Hiroshi Ohno, Shuya Fukai

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Abstract

The tunneling nanotube (TNT) is a structure used for intercellular communication, and is a thin membrane protrusion mediating transport of various signaling molecules and cellular components. M-Sec has potent membrane deformation ability and induces TNT formation in cooperation with the Ral/exocyst complex. Here, we show that the N-terminal polybasic region of M-Sec directly binds phosphatidylinositol (4,5)-bisphosphate for its localization to the plasma membrane during the initial stage of TNT formation. We further report a crystal structure of M-Sec, which consists of helix bundles arranged in a straight rod-like shape, similar to the membrane tethering complex subunits. A positively charged surface in the C-terminal domains is required for M-Sec interaction with active RalA to extend the plasma membrane protrusions. Our results suggest that the membrane-associated M-Sec recruits active RalA, which directs the exocyst complex to form TNTs.

Original languageEnglish
Article number33548
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Sep 15

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nanotubes
membranes
tethering
trinitrotoluene
helices
bundles
rods
communication
crystal structure
molecules
interactions

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Distinct Roles for the N-and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation. / Kimura, Shunsuke; Yamashita, Masami; Yamakami-Kimura, Megumi; Sato, Yusuke; Yamagata, Atsushi; Kobashigawa, Yoshihiro; Inagaki, Fuyuhiko; Amada, Takako; Hase, Kouji; Iwanaga, Toshihiko; Ohno, Hiroshi; Fukai, Shuya.

In: Scientific Reports, Vol. 6, 33548, 15.09.2016.

Research output: Contribution to journalArticle

Kimura, S, Yamashita, M, Yamakami-Kimura, M, Sato, Y, Yamagata, A, Kobashigawa, Y, Inagaki, F, Amada, T, Hase, K, Iwanaga, T, Ohno, H & Fukai, S 2016, 'Distinct Roles for the N-and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation', Scientific Reports, vol. 6, 33548. https://doi.org/10.1038/srep33548
Kimura, Shunsuke ; Yamashita, Masami ; Yamakami-Kimura, Megumi ; Sato, Yusuke ; Yamagata, Atsushi ; Kobashigawa, Yoshihiro ; Inagaki, Fuyuhiko ; Amada, Takako ; Hase, Kouji ; Iwanaga, Toshihiko ; Ohno, Hiroshi ; Fukai, Shuya. / Distinct Roles for the N-and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation. In: Scientific Reports. 2016 ; Vol. 6.
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