Distinct spatio-temporal expression of ABCA and ABCG transporters in the developing and adult mouse brain

Masanori Tachikawa, Masahiko Watanabe, Satoko Hori, Masahiro Fukaya, Sumio Ohtsuki, Tomoko Asashima, Tetsuya Terasaki

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Using in situ hybridization for the mouse brain, we analyzed developmental changes in gene expression for the ATP-binding cassette (ABC) transporter subfamilies ABCA1-4 and 7, and ABCG1, 2, 4, 5 and 8. In the embryonic brains, ABCA1 and A7 were highly expressed in the ventricular (or germinal) zone, whereas ABCA2, A3 and G4 were enriched in the mantle (or differentiating) zone. At the postnatal stages, ABCA1 was detected in both the gray and white matter and in the choroid plexus. On the other hand, ABCA2, A3 and A7 were distributed in the gray matter. In addition, marked up-regulation of ABCA2 occurred in the white matter at 14 days-of-age when various myelin protein genes are known to be up-regulated. In marked contrast, ABCA4 was selective to the choroid plexus throughout development. ABCG1 was expressed in both the gray and white matters, whereas ABCG4 was confined to the gray matter. ABCG2 was diffusely and weakly detected throughout the brain at all stages examined. Immunohistochemistry of ABCG2 showed its preferential expression on the luminal membrane of brain capillaries. Expression signals for ABCG5 and G8 were barely detected at any stages. The distinct spatio-temporal expressions of individual ABCA and G transporters may reflect their distinct cellular expressions in the developing and adult brains, presumably, to regulate and maintain lipid homeostasis in the brain.

Original languageEnglish
Pages (from-to)294-304
Number of pages11
JournalJournal of Neurochemistry
Volume95
Issue number1
DOIs
Publication statusPublished - 2005 Oct 1
Externally publishedYes

Fingerprint

Brain
Choroid Plexus
Myelin Proteins
ATP-Binding Cassette Transporters
Gene expression
In Situ Hybridization
Sub-Family G ATP Binding Cassette Transporter
Homeostasis
Up-Regulation
Genes
Immunohistochemistry
Membranes
Lipids
Gene Expression
Gray Matter
White Matter

Keywords

  • ATP-binding casette transporters
  • Brain development
  • Immunohistochemistry
  • In situ hybridization

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Tachikawa, M., Watanabe, M., Hori, S., Fukaya, M., Ohtsuki, S., Asashima, T., & Terasaki, T. (2005). Distinct spatio-temporal expression of ABCA and ABCG transporters in the developing and adult mouse brain. Journal of Neurochemistry, 95(1), 294-304. https://doi.org/10.1111/j.1471-4159.2005.03369.x

Distinct spatio-temporal expression of ABCA and ABCG transporters in the developing and adult mouse brain. / Tachikawa, Masanori; Watanabe, Masahiko; Hori, Satoko; Fukaya, Masahiro; Ohtsuki, Sumio; Asashima, Tomoko; Terasaki, Tetsuya.

In: Journal of Neurochemistry, Vol. 95, No. 1, 01.10.2005, p. 294-304.

Research output: Contribution to journalArticle

Tachikawa, M, Watanabe, M, Hori, S, Fukaya, M, Ohtsuki, S, Asashima, T & Terasaki, T 2005, 'Distinct spatio-temporal expression of ABCA and ABCG transporters in the developing and adult mouse brain', Journal of Neurochemistry, vol. 95, no. 1, pp. 294-304. https://doi.org/10.1111/j.1471-4159.2005.03369.x
Tachikawa, Masanori ; Watanabe, Masahiko ; Hori, Satoko ; Fukaya, Masahiro ; Ohtsuki, Sumio ; Asashima, Tomoko ; Terasaki, Tetsuya. / Distinct spatio-temporal expression of ABCA and ABCG transporters in the developing and adult mouse brain. In: Journal of Neurochemistry. 2005 ; Vol. 95, No. 1. pp. 294-304.
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