Distinctive subpopulations of the intestinal microbiota are present in women with unexplained chronic anovulation

Hiroyuki Sasaki, Kazuhiro Kawamura, Toshihiro Kawamura, Toshitaka Odamaki, Noriko Katsumata, Jin Zhong Xiao, Nao Suzuki, Mamoru Tanaka

Research output: Contribution to journalArticle

Abstract

Research question: Do gut microbiota associate with the ovulatory cycle in women showing normogonadotrophic anovulation? In humans, the gut microbiota affects diverse physiological functions and dysbiosis (microbial imbalance) may lead to pathological syndromes. However, there is comparatively little information on the relevance of gut microbiota to reproductive functions in women. Here, a group of women with idiopathic chronic anovulation were examined, who do not exhibit any apparent endocrinological disorder, as they are suitable for investigating the relationship between intestinal bacteria and ovulatory disorders. Design: A prospective observational cohort study was performed on two groups of women who did not exhibit apparent endocrinological disorders but showed either irregular menstrual cycles (IMC group) or normal menstrual cycles (controls). The bacterial composition of faeces from rectal swabs from the women was analysed using next-generation sequencing based on bacterial 16SrRNA genes. Results: A metagenomic analysis indicated that the two groups of women had significant differences in 28 bacterial taxa in their faeces. Prevotella-enriched microbiomes were more abundant in the IMC group, whereas Clostridiales, Ruminococcus and Lachnospiraceae (butyrate-producing bacteria) were present at lower levels in the IMC group. Conclusions: Distinctive subpopulations of intestinal microbiota were identified in women with unexplained chronic anovulation. The results indicate that gut microbiota could be associated with ovarian functions.

Original languageEnglish
Pages (from-to)570-578
Number of pages9
JournalReproductive BioMedicine Online
Volume38
Issue number4
DOIs
Publication statusPublished - 2019 Apr 1

Fingerprint

Anovulation
Menstrual Cycle
Feces
Ruminococcus
Dysbiosis
Prevotella
Bacteria
Bacterial Genes
Metagenomics
Butyrates
Microbiota
Gastrointestinal Microbiome
Observational Studies
Cohort Studies
Research

Keywords

  • Gut microbiota
  • Irregular menstrual cycle
  • Normogonadotrophic anovulation
  • Ovulatory disorders

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Developmental Biology

Cite this

Distinctive subpopulations of the intestinal microbiota are present in women with unexplained chronic anovulation. / Sasaki, Hiroyuki; Kawamura, Kazuhiro; Kawamura, Toshihiro; Odamaki, Toshitaka; Katsumata, Noriko; Xiao, Jin Zhong; Suzuki, Nao; Tanaka, Mamoru.

In: Reproductive BioMedicine Online, Vol. 38, No. 4, 01.04.2019, p. 570-578.

Research output: Contribution to journalArticle

Sasaki, Hiroyuki ; Kawamura, Kazuhiro ; Kawamura, Toshihiro ; Odamaki, Toshitaka ; Katsumata, Noriko ; Xiao, Jin Zhong ; Suzuki, Nao ; Tanaka, Mamoru. / Distinctive subpopulations of the intestinal microbiota are present in women with unexplained chronic anovulation. In: Reproductive BioMedicine Online. 2019 ; Vol. 38, No. 4. pp. 570-578.
@article{dff4d64dedf0466aaa9f26978d617c2c,
title = "Distinctive subpopulations of the intestinal microbiota are present in women with unexplained chronic anovulation",
abstract = "Research question: Do gut microbiota associate with the ovulatory cycle in women showing normogonadotrophic anovulation? In humans, the gut microbiota affects diverse physiological functions and dysbiosis (microbial imbalance) may lead to pathological syndromes. However, there is comparatively little information on the relevance of gut microbiota to reproductive functions in women. Here, a group of women with idiopathic chronic anovulation were examined, who do not exhibit any apparent endocrinological disorder, as they are suitable for investigating the relationship between intestinal bacteria and ovulatory disorders. Design: A prospective observational cohort study was performed on two groups of women who did not exhibit apparent endocrinological disorders but showed either irregular menstrual cycles (IMC group) or normal menstrual cycles (controls). The bacterial composition of faeces from rectal swabs from the women was analysed using next-generation sequencing based on bacterial 16SrRNA genes. Results: A metagenomic analysis indicated that the two groups of women had significant differences in 28 bacterial taxa in their faeces. Prevotella-enriched microbiomes were more abundant in the IMC group, whereas Clostridiales, Ruminococcus and Lachnospiraceae (butyrate-producing bacteria) were present at lower levels in the IMC group. Conclusions: Distinctive subpopulations of intestinal microbiota were identified in women with unexplained chronic anovulation. The results indicate that gut microbiota could be associated with ovarian functions.",
keywords = "Gut microbiota, Irregular menstrual cycle, Normogonadotrophic anovulation, Ovulatory disorders",
author = "Hiroyuki Sasaki and Kazuhiro Kawamura and Toshihiro Kawamura and Toshitaka Odamaki and Noriko Katsumata and Xiao, {Jin Zhong} and Nao Suzuki and Mamoru Tanaka",
year = "2019",
month = "4",
day = "1",
doi = "10.1016/j.rbmo.2018.12.026",
language = "English",
volume = "38",
pages = "570--578",
journal = "Reproductive BioMedicine Online",
issn = "1472-6483",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Distinctive subpopulations of the intestinal microbiota are present in women with unexplained chronic anovulation

AU - Sasaki, Hiroyuki

AU - Kawamura, Kazuhiro

AU - Kawamura, Toshihiro

AU - Odamaki, Toshitaka

AU - Katsumata, Noriko

AU - Xiao, Jin Zhong

AU - Suzuki, Nao

AU - Tanaka, Mamoru

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Research question: Do gut microbiota associate with the ovulatory cycle in women showing normogonadotrophic anovulation? In humans, the gut microbiota affects diverse physiological functions and dysbiosis (microbial imbalance) may lead to pathological syndromes. However, there is comparatively little information on the relevance of gut microbiota to reproductive functions in women. Here, a group of women with idiopathic chronic anovulation were examined, who do not exhibit any apparent endocrinological disorder, as they are suitable for investigating the relationship between intestinal bacteria and ovulatory disorders. Design: A prospective observational cohort study was performed on two groups of women who did not exhibit apparent endocrinological disorders but showed either irregular menstrual cycles (IMC group) or normal menstrual cycles (controls). The bacterial composition of faeces from rectal swabs from the women was analysed using next-generation sequencing based on bacterial 16SrRNA genes. Results: A metagenomic analysis indicated that the two groups of women had significant differences in 28 bacterial taxa in their faeces. Prevotella-enriched microbiomes were more abundant in the IMC group, whereas Clostridiales, Ruminococcus and Lachnospiraceae (butyrate-producing bacteria) were present at lower levels in the IMC group. Conclusions: Distinctive subpopulations of intestinal microbiota were identified in women with unexplained chronic anovulation. The results indicate that gut microbiota could be associated with ovarian functions.

AB - Research question: Do gut microbiota associate with the ovulatory cycle in women showing normogonadotrophic anovulation? In humans, the gut microbiota affects diverse physiological functions and dysbiosis (microbial imbalance) may lead to pathological syndromes. However, there is comparatively little information on the relevance of gut microbiota to reproductive functions in women. Here, a group of women with idiopathic chronic anovulation were examined, who do not exhibit any apparent endocrinological disorder, as they are suitable for investigating the relationship between intestinal bacteria and ovulatory disorders. Design: A prospective observational cohort study was performed on two groups of women who did not exhibit apparent endocrinological disorders but showed either irregular menstrual cycles (IMC group) or normal menstrual cycles (controls). The bacterial composition of faeces from rectal swabs from the women was analysed using next-generation sequencing based on bacterial 16SrRNA genes. Results: A metagenomic analysis indicated that the two groups of women had significant differences in 28 bacterial taxa in their faeces. Prevotella-enriched microbiomes were more abundant in the IMC group, whereas Clostridiales, Ruminococcus and Lachnospiraceae (butyrate-producing bacteria) were present at lower levels in the IMC group. Conclusions: Distinctive subpopulations of intestinal microbiota were identified in women with unexplained chronic anovulation. The results indicate that gut microbiota could be associated with ovarian functions.

KW - Gut microbiota

KW - Irregular menstrual cycle

KW - Normogonadotrophic anovulation

KW - Ovulatory disorders

UR - http://www.scopus.com/inward/record.url?scp=85061444347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061444347&partnerID=8YFLogxK

U2 - 10.1016/j.rbmo.2018.12.026

DO - 10.1016/j.rbmo.2018.12.026

M3 - Article

C2 - 30773302

AN - SCOPUS:85061444347

VL - 38

SP - 570

EP - 578

JO - Reproductive BioMedicine Online

JF - Reproductive BioMedicine Online

SN - 1472-6483

IS - 4

ER -