Distinguishing the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical quenching effect from the hydroxyl radical scavenging effect in the ESR spin-trapping method

Linxiang Li; Yoshihiro Abe; Kiyotada Kanagawa; Noriko Usui; Kazuhiro Imai; Tadahiko Mashino; Masataka Mochizuki; Naoki Miyata

doi:10.1016/j.aca.2004.02.020

Distinguishing the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical quenching effect from the hydroxyl radical scavenging effect in the ESR spin-trapping method

Linxiang Li, Yoshihiro Abe, Kiyotada Kanagawa, Noriko Usui, Kazuhiro Imai, Tadahiko Mashino, Masataka Mochizuki, Naoki Miyata

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79 Citations (Scopus)

Abstract

The spin-trapping method has been widely accepted to measure the hydroxyl radical scavenging activity in electron spin resonance (ESR) spectroscopy, however, the disappearance of the trapped signal of the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical may lead to an erroneous conclusion, as the decreased signal must result from the rapid quenching of the DMPO-OH formed. The DMPO-OH quenching activity of a compound can be measured by tracing the signal decay resulting from its addition after the cessation of DMPO-OH formation. It was found that Fe(II) ions could quench the DMPO-OH radical quite rapidly, and phosphate ions could enhance the DMPO-OH radical quenching activity of the Fe(II) ions.

Original language	English
Pages (from-to)	121-124
Number of pages	4
Journal	Analytica Chimica Acta
Volume	512
Issue number	1
DOIs	https://doi.org/10.1016/j.aca.2004.02.020
Publication status	Published - 2004 Jun 4

Keywords

DMPO
ESR
Fenton reaction
Hydroxyl radical
Spin-trapping

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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title = "Distinguishing the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical quenching effect from the hydroxyl radical scavenging effect in the ESR spin-trapping method",

abstract = "The spin-trapping method has been widely accepted to measure the hydroxyl radical scavenging activity in electron spin resonance (ESR) spectroscopy, however, the disappearance of the trapped signal of the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical may lead to an erroneous conclusion, as the decreased signal must result from the rapid quenching of the DMPO-OH formed. The DMPO-OH quenching activity of a compound can be measured by tracing the signal decay resulting from its addition after the cessation of DMPO-OH formation. It was found that Fe(II) ions could quench the DMPO-OH radical quite rapidly, and phosphate ions could enhance the DMPO-OH radical quenching activity of the Fe(II) ions.",

keywords = "DMPO, ESR, Fenton reaction, Hydroxyl radical, Spin-trapping",

author = "Linxiang Li and Yoshihiro Abe and Kiyotada Kanagawa and Noriko Usui and Kazuhiro Imai and Tadahiko Mashino and Masataka Mochizuki and Naoki Miyata",

year = "2004",

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doi = "10.1016/j.aca.2004.02.020",

language = "English",

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T1 - Distinguishing the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical quenching effect from the hydroxyl radical scavenging effect in the ESR spin-trapping method

AU - Li, Linxiang

AU - Abe, Yoshihiro

AU - Kanagawa, Kiyotada

AU - Usui, Noriko

AU - Imai, Kazuhiro

AU - Mashino, Tadahiko

AU - Mochizuki, Masataka

AU - Miyata, Naoki

PY - 2004/6/4

Y1 - 2004/6/4

N2 - The spin-trapping method has been widely accepted to measure the hydroxyl radical scavenging activity in electron spin resonance (ESR) spectroscopy, however, the disappearance of the trapped signal of the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical may lead to an erroneous conclusion, as the decreased signal must result from the rapid quenching of the DMPO-OH formed. The DMPO-OH quenching activity of a compound can be measured by tracing the signal decay resulting from its addition after the cessation of DMPO-OH formation. It was found that Fe(II) ions could quench the DMPO-OH radical quite rapidly, and phosphate ions could enhance the DMPO-OH radical quenching activity of the Fe(II) ions.

AB - The spin-trapping method has been widely accepted to measure the hydroxyl radical scavenging activity in electron spin resonance (ESR) spectroscopy, however, the disappearance of the trapped signal of the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical may lead to an erroneous conclusion, as the decreased signal must result from the rapid quenching of the DMPO-OH formed. The DMPO-OH quenching activity of a compound can be measured by tracing the signal decay resulting from its addition after the cessation of DMPO-OH formation. It was found that Fe(II) ions could quench the DMPO-OH radical quite rapidly, and phosphate ions could enhance the DMPO-OH radical quenching activity of the Fe(II) ions.

KW - DMPO

KW - ESR

KW - Fenton reaction

KW - Hydroxyl radical

KW - Spin-trapping

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JO - Analytica Chimica Acta

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ER -

Distinguishing the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical quenching effect from the hydroxyl radical scavenging effect in the ESR spin-trapping method

Abstract

Keywords

ASJC Scopus subject areas

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