Distinguishing the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical quenching effect from the hydroxyl radical scavenging effect in the ESR spin-trapping method

Linxiang Li, Yoshihiro Abe, Kiyotada Kanagawa, Noriko Usui, Kazuhiro Imai, Tadahiko Mashino, Masataka Mochizuki, Naoki Miyata

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The spin-trapping method has been widely accepted to measure the hydroxyl radical scavenging activity in electron spin resonance (ESR) spectroscopy, however, the disappearance of the trapped signal of the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical may lead to an erroneous conclusion, as the decreased signal must result from the rapid quenching of the DMPO-OH formed. The DMPO-OH quenching activity of a compound can be measured by tracing the signal decay resulting from its addition after the cessation of DMPO-OH formation. It was found that Fe(II) ions could quench the DMPO-OH radical quite rapidly, and phosphate ions could enhance the DMPO-OH radical quenching activity of the Fe(II) ions.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalAnalytica Chimica Acta
Volume512
Issue number1
DOIs
Publication statusPublished - 2004 Jun 4

Fingerprint

Spin Trapping
Scavenging
Electron Spin Resonance Spectroscopy
electron spin resonance
hydroxyl radical
Hydroxyl Radical
Paramagnetic resonance
trapping
Quenching
oxide
Ions
ion
Electron spin resonance spectroscopy
Rapid quenching
5,5-dimethyl-1-pyrroline-1-oxide
effect
method
Phosphates
spectroscopy
phosphate

Keywords

  • DMPO
  • ESR
  • Fenton reaction
  • Hydroxyl radical
  • Spin-trapping

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Distinguishing the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical quenching effect from the hydroxyl radical scavenging effect in the ESR spin-trapping method. / Li, Linxiang; Abe, Yoshihiro; Kanagawa, Kiyotada; Usui, Noriko; Imai, Kazuhiro; Mashino, Tadahiko; Mochizuki, Masataka; Miyata, Naoki.

In: Analytica Chimica Acta, Vol. 512, No. 1, 04.06.2004, p. 121-124.

Research output: Contribution to journalArticle

Li, Linxiang ; Abe, Yoshihiro ; Kanagawa, Kiyotada ; Usui, Noriko ; Imai, Kazuhiro ; Mashino, Tadahiko ; Mochizuki, Masataka ; Miyata, Naoki. / Distinguishing the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical quenching effect from the hydroxyl radical scavenging effect in the ESR spin-trapping method. In: Analytica Chimica Acta. 2004 ; Vol. 512, No. 1. pp. 121-124.
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AU - Abe, Yoshihiro

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