Distribution and function of hyaluronan binding protein involved in hyaluronan depolymerization (HYBID, KIAA1199) in the mouse central nervous system

Yuta Yoshino, Mitsue Ishisaka, Kazuhiro Tsuruma, Masamitsu Shimazawa, Hiroyuki Yoshida, Shintaro Inoue, Masayuki Shimoda, Yasunori Okada, Hideaki Hara

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

HYBID (HYaluronan Binding Protein Involved in hyaluronan [HA] Depolymerization, KIAA1199) is one of the HA binding proteins that is involved in the depolymerization of HA. HYBID mRNA is highly expressed in the brain, however, the role of HYBID in the brain remains unclear. In this study, we bred Hybid knock-out (KO) mice and evaluated the function of Hybid in the central nervous system. Hybid mRNA was expressed in the brain, especially in the hippocampus and cerebellum, in wild-type mice. Hybid KO mice demonstrated decreased mnemonic ability in novel object recognition and Morris water maze tests. The average molecular mass of hippocampal HA increased in KO mice, accompanied by a significant increase in the total HA amount. Hybid KO mice did not differ in behavior from wild-type mice in the open field test, evaluation of acoustic startle responses, or drug-induced seizure test. In real-time PCR, Hyal1 and Hyal2 mRNA levels, which code hyaluronidases 1 and 2, respectively, did not differ between the Hybid KO and wild-type mouse brain. These results indicate that Hybid plays a key role in memory function in the brain.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalNeuroscience
Volume347
DOIs
Publication statusPublished - 2017 Apr 7

Fingerprint

CD44 Antigens
Hyaluronic Acid
Knockout Mice
Central Nervous System
Brain
Messenger RNA
Startle Reflex
Aptitude
Hyaluronoglucosaminidase
Acoustics
Cerebellum
Real-Time Polymerase Chain Reaction
Hippocampus
Seizures
Water
Pharmaceutical Preparations

Keywords

  • behavioral test
  • cerebellum
  • hippocampus
  • hyaluronan
  • HYBID
  • memory

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distribution and function of hyaluronan binding protein involved in hyaluronan depolymerization (HYBID, KIAA1199) in the mouse central nervous system. / Yoshino, Yuta; Ishisaka, Mitsue; Tsuruma, Kazuhiro; Shimazawa, Masamitsu; Yoshida, Hiroyuki; Inoue, Shintaro; Shimoda, Masayuki; Okada, Yasunori; Hara, Hideaki.

In: Neuroscience, Vol. 347, 07.04.2017, p. 1-10.

Research output: Contribution to journalArticle

Yoshino, Yuta ; Ishisaka, Mitsue ; Tsuruma, Kazuhiro ; Shimazawa, Masamitsu ; Yoshida, Hiroyuki ; Inoue, Shintaro ; Shimoda, Masayuki ; Okada, Yasunori ; Hara, Hideaki. / Distribution and function of hyaluronan binding protein involved in hyaluronan depolymerization (HYBID, KIAA1199) in the mouse central nervous system. In: Neuroscience. 2017 ; Vol. 347. pp. 1-10.
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