Distribution of the high-affinity choline transporter in the human and macaque monkey spinal cord

Yasushi Kobayashi, Takashi Okuda, Yasunori Fujioka, George Matsumura, Yu Nishimura, Tatsuya Haga

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The distribution of the high-affinity choline transporter (CHT) was determined in the human and macaque monkey spinal cord using in situ hybridization histochemistry and immunohistochemistry. Signals for CHT mRNA were observed in somatic motor neurons, sympathetic preganglionic neurons, and neurons in the medial part of lamina VII. The mRNA for CHT was co-localized in single neurons with the mRNAs for vesicular acetylcholine transporter and cholineacetyltransferase. These same cholinergic neuronal groups were labeled by immunohistochemistry for human CHT. Of somatic motor neurons, smaller cell bodies of γ-motor neurons were labeled very intensely, whereas larger cell bodies of α-motor neurons showed various degrees of labeling from weak to moderately intense. Human CHT is thus a novel cholinergic marker, which not only labels cholinergic neurons, but also reveals their heterogeneity.

Original languageEnglish
Pages (from-to)25-28
Number of pages4
JournalNeuroscience Letters
Volume317
Issue number1
DOIs
Publication statusPublished - 2002 Jan 4
Externally publishedYes

Fingerprint

Macaca
Haplorhini
Motor Neurons
Spinal Cord
Neurons
Messenger RNA
Cholinergic Agents
Vesicular Acetylcholine Transport Proteins
Immunohistochemistry
Cholinergic Neurons
In Situ Hybridization
choline transporter
Cell Body

Keywords

  • Choline acetyltransferase
  • High-affinity choline trasnporter
  • Immunohistochemistry
  • In situ hybridization
  • Motor neurons
  • Vesicular acetylcholine trasnporter

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distribution of the high-affinity choline transporter in the human and macaque monkey spinal cord. / Kobayashi, Yasushi; Okuda, Takashi; Fujioka, Yasunori; Matsumura, George; Nishimura, Yu; Haga, Tatsuya.

In: Neuroscience Letters, Vol. 317, No. 1, 04.01.2002, p. 25-28.

Research output: Contribution to journalArticle

Kobayashi, Yasushi ; Okuda, Takashi ; Fujioka, Yasunori ; Matsumura, George ; Nishimura, Yu ; Haga, Tatsuya. / Distribution of the high-affinity choline transporter in the human and macaque monkey spinal cord. In: Neuroscience Letters. 2002 ; Vol. 317, No. 1. pp. 25-28.
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