TY - JOUR
T1 - Distribution of the high-affinity choline transporter in the human and macaque monkey spinal cord
AU - Kobayashi, Yasushi
AU - Okuda, Takashi
AU - Fujioka, Yasunori
AU - Matsumura, George
AU - Nishimura, Yu
AU - Haga, Tatsuya
N1 - Funding Information:
We thank Drs A. Nanbu, H. Tokuno and N. Ishizuka (Tokyo Metropolitan Institute for Neuroscience) for providing monkey spinal cord tissue, and Dr D.G. Amaral (Department of Psychiatry and Center for Neuroscience, University of California, Davis) for his valuable advice concerning the in situ hybridization techniques. This work was supported by grants from the Japan Science and Technology Corporation (CREST), the Ministry of Japan Society for the Promotion of Science (Research for the Future Program) and the Promotion and Mutual Aid Corporation of Private Schools of Japan.
PY - 2002/1/4
Y1 - 2002/1/4
N2 - The distribution of the high-affinity choline transporter (CHT) was determined in the human and macaque monkey spinal cord using in situ hybridization histochemistry and immunohistochemistry. Signals for CHT mRNA were observed in somatic motor neurons, sympathetic preganglionic neurons, and neurons in the medial part of lamina VII. The mRNA for CHT was co-localized in single neurons with the mRNAs for vesicular acetylcholine transporter and cholineacetyltransferase. These same cholinergic neuronal groups were labeled by immunohistochemistry for human CHT. Of somatic motor neurons, smaller cell bodies of γ-motor neurons were labeled very intensely, whereas larger cell bodies of α-motor neurons showed various degrees of labeling from weak to moderately intense. Human CHT is thus a novel cholinergic marker, which not only labels cholinergic neurons, but also reveals their heterogeneity.
AB - The distribution of the high-affinity choline transporter (CHT) was determined in the human and macaque monkey spinal cord using in situ hybridization histochemistry and immunohistochemistry. Signals for CHT mRNA were observed in somatic motor neurons, sympathetic preganglionic neurons, and neurons in the medial part of lamina VII. The mRNA for CHT was co-localized in single neurons with the mRNAs for vesicular acetylcholine transporter and cholineacetyltransferase. These same cholinergic neuronal groups were labeled by immunohistochemistry for human CHT. Of somatic motor neurons, smaller cell bodies of γ-motor neurons were labeled very intensely, whereas larger cell bodies of α-motor neurons showed various degrees of labeling from weak to moderately intense. Human CHT is thus a novel cholinergic marker, which not only labels cholinergic neurons, but also reveals their heterogeneity.
KW - Choline acetyltransferase
KW - High-affinity choline trasnporter
KW - Immunohistochemistry
KW - In situ hybridization
KW - Motor neurons
KW - Vesicular acetylcholine trasnporter
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U2 - 10.1016/S0304-3940(01)02413-2
DO - 10.1016/S0304-3940(01)02413-2
M3 - Article
C2 - 11750988
AN - SCOPUS:0037016513
VL - 317
SP - 25
EP - 28
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -