Abstract
The distribution of the high-affinity choline transporter (CHT) was determined in the human and macaque monkey spinal cord using in situ hybridization histochemistry and immunohistochemistry. Signals for CHT mRNA were observed in somatic motor neurons, sympathetic preganglionic neurons, and neurons in the medial part of lamina VII. The mRNA for CHT was co-localized in single neurons with the mRNAs for vesicular acetylcholine transporter and cholineacetyltransferase. These same cholinergic neuronal groups were labeled by immunohistochemistry for human CHT. Of somatic motor neurons, smaller cell bodies of γ-motor neurons were labeled very intensely, whereas larger cell bodies of α-motor neurons showed various degrees of labeling from weak to moderately intense. Human CHT is thus a novel cholinergic marker, which not only labels cholinergic neurons, but also reveals their heterogeneity.
| Original language | English |
|---|---|
| Pages (from-to) | 25-28 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 317 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2002 Jan 4 |
| Externally published | Yes |
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Keywords
- Choline acetyltransferase
- High-affinity choline trasnporter
- Immunohistochemistry
- In situ hybridization
- Motor neurons
- Vesicular acetylcholine trasnporter
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Distribution of the high-affinity choline transporter in the human and macaque monkey spinal cord. / Kobayashi, Yasushi; Okuda, Takashi; Fujioka, Yasunori; Matsumura, George; Nishimura, Yu; Haga, Tatsuya.
In: Neuroscience Letters, Vol. 317, No. 1, 04.01.2002, p. 25-28.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Distribution of the high-affinity choline transporter in the human and macaque monkey spinal cord
AU - Kobayashi, Yasushi
AU - Okuda, Takashi
AU - Fujioka, Yasunori
AU - Matsumura, George
AU - Nishimura, Yu
AU - Haga, Tatsuya
PY - 2002/1/4
Y1 - 2002/1/4
N2 - The distribution of the high-affinity choline transporter (CHT) was determined in the human and macaque monkey spinal cord using in situ hybridization histochemistry and immunohistochemistry. Signals for CHT mRNA were observed in somatic motor neurons, sympathetic preganglionic neurons, and neurons in the medial part of lamina VII. The mRNA for CHT was co-localized in single neurons with the mRNAs for vesicular acetylcholine transporter and cholineacetyltransferase. These same cholinergic neuronal groups were labeled by immunohistochemistry for human CHT. Of somatic motor neurons, smaller cell bodies of γ-motor neurons were labeled very intensely, whereas larger cell bodies of α-motor neurons showed various degrees of labeling from weak to moderately intense. Human CHT is thus a novel cholinergic marker, which not only labels cholinergic neurons, but also reveals their heterogeneity.
AB - The distribution of the high-affinity choline transporter (CHT) was determined in the human and macaque monkey spinal cord using in situ hybridization histochemistry and immunohistochemistry. Signals for CHT mRNA were observed in somatic motor neurons, sympathetic preganglionic neurons, and neurons in the medial part of lamina VII. The mRNA for CHT was co-localized in single neurons with the mRNAs for vesicular acetylcholine transporter and cholineacetyltransferase. These same cholinergic neuronal groups were labeled by immunohistochemistry for human CHT. Of somatic motor neurons, smaller cell bodies of γ-motor neurons were labeled very intensely, whereas larger cell bodies of α-motor neurons showed various degrees of labeling from weak to moderately intense. Human CHT is thus a novel cholinergic marker, which not only labels cholinergic neurons, but also reveals their heterogeneity.
KW - Choline acetyltransferase
KW - High-affinity choline trasnporter
KW - Immunohistochemistry
KW - In situ hybridization
KW - Motor neurons
KW - Vesicular acetylcholine trasnporter
UR - http://www.scopus.com/inward/record.url?scp=0037016513&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037016513&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(01)02413-2
DO - 10.1016/S0304-3940(01)02413-2
M3 - Article
C2 - 11750988
AN - SCOPUS:0037016513
VL - 317
SP - 25
EP - 28
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -