Diverse mutations in the ftsI gene in ampicillin-resistant Haemophilus influenzae isolates from pediatric patients with acute otitis media

Kozue Kishii, Naoko Chiba, Miyuki Morozumi, Keiko Hamano-Hasegawa, Iku Kurokawa, Junko Masaki, Kimiko Ubukata

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

To clarify molecular changes in β-lactamase-nonproducing, ampicillin-resistant (BLNAR) Haemophilus influenzae, which is increasing in pediatric patients with acute otitis media (AOM) in Japan, we identified amino acid (aa) substitutions in penicillin-binding protein 3 for the BLNAR strains. Of 191 H. influenzae strains isolated from middle ear fluid of pediatric AOM patients between October 2005 and March 2008, BLNAR strains determined by PCR accounted for 49.2%. Of the BLNAR strains, 91.5% possessed 4 aa substitutions: Met377Ile, Ser385Thr, Leu389Phe, and either Asn526Lys or Arg517His. Additionally, the emergence of BLNAR strains possessing a new aa substitution of Val329Ala in the conserved aa motif of Ser327-Thr-Val-Lys, or Val511Ala adjacent to the conserved aa motif of Lys512-Thr-Gly, was noted. Transformation of the ftsI gene into the Rd reference strain (ATCC 51907) demonstrated that these two aa substitutions reduced susceptibility to amoxicillin more than to cephalosporins. Pulsed-field gel electrophoretic profiles of BLNAR strains were highly diverse. These results suggested that inadequate antibiotic use may increase BLNAR strains by selecting mutations in the ftsI gene and that such use may have favored the new aa substitutions.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalJournal of Infection and Chemotherapy
Volume16
Issue number2
DOIs
Publication statusPublished - 2010 Apr
Externally publishedYes

Fingerprint

Haemophilus influenzae
Otitis Media
Amino Acid Substitution
Ampicillin
Pediatrics
Mutation
Amino Acid Motifs
Genes
threonyl-glycine
Penicillin-Binding Proteins
Amoxicillin
Middle Ear
Cephalosporins
Japan
Gels
Anti-Bacterial Agents
Polymerase Chain Reaction

Keywords

  • β-Lactamase-nonproducing
  • Ampicillin-resistant (BLNAR)
  • Haemophilus influenzae
  • Otitis media
  • Pulsed-field gel electrophoresis (PFGE)

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Diverse mutations in the ftsI gene in ampicillin-resistant Haemophilus influenzae isolates from pediatric patients with acute otitis media. / Kishii, Kozue; Chiba, Naoko; Morozumi, Miyuki; Hamano-Hasegawa, Keiko; Kurokawa, Iku; Masaki, Junko; Ubukata, Kimiko.

In: Journal of Infection and Chemotherapy, Vol. 16, No. 2, 04.2010, p. 87-93.

Research output: Contribution to journalArticle

Kishii, Kozue ; Chiba, Naoko ; Morozumi, Miyuki ; Hamano-Hasegawa, Keiko ; Kurokawa, Iku ; Masaki, Junko ; Ubukata, Kimiko. / Diverse mutations in the ftsI gene in ampicillin-resistant Haemophilus influenzae isolates from pediatric patients with acute otitis media. In: Journal of Infection and Chemotherapy. 2010 ; Vol. 16, No. 2. pp. 87-93.
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