Diversity of mRNA expression for muscarinic acetylcholine receptor subtypes and neuronal nicotinic acetylcholine receptor subunits in human mononuclear leukocytes and leukemic cell lines

Kazuo Z. Sato, Takeshi Fujii, Yoshihiro Watanabe, Shin Yamada, Tsuyoshi Ando, Kazuko Fujimoto, Koichiro Kawashima

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

Previously, we reported that various levels of acetylcholine (ACh), currently known as a neurotransmitter, are detectable in the blood of several mammals including humans and that most blood ACh originates from T- lymphocytes. To investigate whether ACh in the blood acts on lymphocytes and participates in the modulation of immune responses, we have analyzed the expression of mRNA for muscarinic (Ms) ACh receptor subtypes and nicotinic (Nc) ACh receptor subunits using reverse transcription-polymerase chain reaction (RT-PCR) methods. The cells tested were human peripheral mononuclear leukocytes (MNLs) from seven healthy donors and eight leukemic cell lines, as models of lymphocytes. We detected mRNA expression for various neuronal Nc receptor subunits and Ms receptor subtypes in all of the MNL samples and in all of the cell lines tested. However, the expression pattern of mRNA for neuronal Nc receptor subunits (α2-α7 and β2-β4) and Ms receptor subtypes (m1-m5) varied among the individuals and cell lines. No expression of mRNA for three muscle-type Nc receptor subunits (α1, β1 and γ) was observed in the MNLs and cell lines. These results indicate that both neuronal-type Nc and Ms ACh receptors are present on the surface of lymphocytes.

Original languageEnglish
Pages (from-to)17-20
Number of pages4
JournalNeuroscience Letters
Volume266
Issue number1
DOIs
Publication statusPublished - 1999 Apr 30

Fingerprint

Mononuclear Leukocytes
Nicotinic Receptors
Muscarinic Receptors
Cell Line
Messenger RNA
Acetylcholine
Lymphocytes
Muscarinic M1 Receptors
Reverse Transcription
Neurotransmitter Agents
Mammals
T-Lymphocytes
Muscles
Polymerase Chain Reaction

Keywords

  • Acetylcholine
  • B-lymphocyte
  • Muscarinic receptor
  • Nicotinic receptor
  • Reverse transcription-polymerase chain reaction
  • T-lymphocyte

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Diversity of mRNA expression for muscarinic acetylcholine receptor subtypes and neuronal nicotinic acetylcholine receptor subunits in human mononuclear leukocytes and leukemic cell lines. / Sato, Kazuo Z.; Fujii, Takeshi; Watanabe, Yoshihiro; Yamada, Shin; Ando, Tsuyoshi; Fujimoto, Kazuko; Kawashima, Koichiro.

In: Neuroscience Letters, Vol. 266, No. 1, 30.04.1999, p. 17-20.

Research output: Contribution to journalArticle

Sato, Kazuo Z. ; Fujii, Takeshi ; Watanabe, Yoshihiro ; Yamada, Shin ; Ando, Tsuyoshi ; Fujimoto, Kazuko ; Kawashima, Koichiro. / Diversity of mRNA expression for muscarinic acetylcholine receptor subtypes and neuronal nicotinic acetylcholine receptor subunits in human mononuclear leukocytes and leukemic cell lines. In: Neuroscience Letters. 1999 ; Vol. 266, No. 1. pp. 17-20.
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