DmGTSF1 is necessary for Piwi-piRISC-mediated transcriptional transposon silencing in the Drosophila ovary

Hitoshi Ohtani, Yuka W. Iwasaki, Aoi Shibuya, Haruhiko Siomi, Mikiko C. Siomi, Kuniaki Saito

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The Piwi-piRNA (PIWI-interacting RNA) complex (Piwi- piRISC) in Drosophila ovarian somatic cells represses transposons transcriptionally to maintain genome integrity; however, the underlying mechanisms remain obscure. Here, we reveal that DmGTSF1, a Drosophila homolog of gametocyte-specific factor 1 (GTSF1) (which is required for transposon silencing in mouse testes), is necessary for Piwi-piRISC to repress target transposons and neighboring genes. DmGTSF1 depletion affected neither piRNA biogenesis nor nuclear import of Piwi- piRISC. DmGTSF1 mutations caused derepression of transposons and loss of ovary follicle layers, resulting in female infertility. We suggest that DmGTSF1, a nuclear Piwi interactor, is an integral factor in Piwi-piRISCmediated transcriptional silencing.

Original languageEnglish
Pages (from-to)1656-1661
Number of pages6
JournalGenes and Development
Volume27
Issue number15
DOIs
Publication statusPublished - 2013 Aug 1

Keywords

  • DmGTSF1
  • Drosophila
  • Piwi
  • RNA silencing
  • Transposon
  • piRNA

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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