DMPO-OH radical formation from 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in hot water

Tomoko Shoji, Linxiang Li, Yoshihiro Abe, Masahiro Ogata, Yoshihisa Ishimoto, Ryoko Gonda, Tadahiko Mashino, Masataka Mochizuki, Michihisa Uemoto, Naoki Miyata

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

When an aqueous solution of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was heated at 70°C for 30 min, formation of DMPO-OH was observed by ESR. This DMPO-OH radical formation was suppressed under an argon atmosphere. When water was replaced with ultra-pure water for ICP-MS experiments, DMPO-OH radical formation was also diminished. Under an argon atmosphere in ultra-pure water, the intensity of the DMPO-OH signal decreased to about 1/20 of that observed under aerobic conditions with regular purified water. The addition of hydroxyl radical scavengers such as mannitol did not affect the formation of DMPO-OH, but the signal turned faint in the presence of EDTA. We suggest that DMPO reacted with dissolved oxygen to form DMPO-OH. 2007

Original languageEnglish
Pages (from-to)219-221
Number of pages3
JournalAnalytical Sciences
Volume23
Issue number2
DOIs
Publication statusPublished - 2007 Feb

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Water
Argon
5,5-dimethyl-1-pyrroline-1-oxide
Mannitol
Dissolved oxygen
Edetic Acid
Hydroxyl Radical
Paramagnetic resonance
Experiments

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

DMPO-OH radical formation from 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in hot water. / Shoji, Tomoko; Li, Linxiang; Abe, Yoshihiro; Ogata, Masahiro; Ishimoto, Yoshihisa; Gonda, Ryoko; Mashino, Tadahiko; Mochizuki, Masataka; Uemoto, Michihisa; Miyata, Naoki.

In: Analytical Sciences, Vol. 23, No. 2, 02.2007, p. 219-221.

Research output: Contribution to journalArticle

Shoji, T, Li, L, Abe, Y, Ogata, M, Ishimoto, Y, Gonda, R, Mashino, T, Mochizuki, M, Uemoto, M & Miyata, N 2007, 'DMPO-OH radical formation from 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in hot water', Analytical Sciences, vol. 23, no. 2, pp. 219-221. https://doi.org/10.2116/analsci.23.219
Shoji, Tomoko ; Li, Linxiang ; Abe, Yoshihiro ; Ogata, Masahiro ; Ishimoto, Yoshihisa ; Gonda, Ryoko ; Mashino, Tadahiko ; Mochizuki, Masataka ; Uemoto, Michihisa ; Miyata, Naoki. / DMPO-OH radical formation from 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in hot water. In: Analytical Sciences. 2007 ; Vol. 23, No. 2. pp. 219-221.
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AU - Gonda, Ryoko

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