DMPO-OH radical formation from 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in hot water

Tomoko Shoji; Linxiang Li; Yoshihiro Abe; Masahiro Ogata; Yoshihisa Ishimoto; Ryoko Gonda; Tadahiko Mashino; Masataka Mochizuki; Michihisa Uemoto; Naoki Miyata

doi:10.2116/analsci.23.219

DMPO-OH radical formation from 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in hot water

Tomoko Shoji, Linxiang Li, Yoshihiro Abe, Masahiro Ogata, Yoshihisa Ishimoto, Ryoko Gonda, Tadahiko Mashino, Masataka Mochizuki, Michihisa Uemoto, Naoki Miyata

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

When an aqueous solution of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was heated at 70°C for 30 min, formation of DMPO-OH was observed by ESR. This DMPO-OH radical formation was suppressed under an argon atmosphere. When water was replaced with ultra-pure water for ICP-MS experiments, DMPO-OH radical formation was also diminished. Under an argon atmosphere in ultra-pure water, the intensity of the DMPO-OH signal decreased to about 1/20 of that observed under aerobic conditions with regular purified water. The addition of hydroxyl radical scavengers such as mannitol did not affect the formation of DMPO-OH, but the signal turned faint in the presence of EDTA. We suggest that DMPO reacted with dissolved oxygen to form DMPO-OH. 2007

Original language	English
Pages (from-to)	219-221
Number of pages	3
Journal	analytical sciences
Volume	23
Issue number	2
DOIs	https://doi.org/10.2116/analsci.23.219
Publication status	Published - 2007 Feb

ASJC Scopus subject areas

Analytical Chemistry

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title = "DMPO-OH radical formation from 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in hot water",

abstract = "When an aqueous solution of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was heated at 70°C for 30 min, formation of DMPO-OH was observed by ESR. This DMPO-OH radical formation was suppressed under an argon atmosphere. When water was replaced with ultra-pure water for ICP-MS experiments, DMPO-OH radical formation was also diminished. Under an argon atmosphere in ultra-pure water, the intensity of the DMPO-OH signal decreased to about 1/20 of that observed under aerobic conditions with regular purified water. The addition of hydroxyl radical scavengers such as mannitol did not affect the formation of DMPO-OH, but the signal turned faint in the presence of EDTA. We suggest that DMPO reacted with dissolved oxygen to form DMPO-OH. 2007",

author = "Tomoko Shoji and Linxiang Li and Yoshihiro Abe and Masahiro Ogata and Yoshihisa Ishimoto and Ryoko Gonda and Tadahiko Mashino and Masataka Mochizuki and Michihisa Uemoto and Naoki Miyata",

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AU - Shoji, Tomoko

AU - Li, Linxiang

AU - Abe, Yoshihiro

AU - Ogata, Masahiro

AU - Ishimoto, Yoshihisa

AU - Gonda, Ryoko

AU - Mashino, Tadahiko

AU - Mochizuki, Masataka

AU - Uemoto, Michihisa

AU - Miyata, Naoki

PY - 2007/2

Y1 - 2007/2

N2 - When an aqueous solution of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was heated at 70°C for 30 min, formation of DMPO-OH was observed by ESR. This DMPO-OH radical formation was suppressed under an argon atmosphere. When water was replaced with ultra-pure water for ICP-MS experiments, DMPO-OH radical formation was also diminished. Under an argon atmosphere in ultra-pure water, the intensity of the DMPO-OH signal decreased to about 1/20 of that observed under aerobic conditions with regular purified water. The addition of hydroxyl radical scavengers such as mannitol did not affect the formation of DMPO-OH, but the signal turned faint in the presence of EDTA. We suggest that DMPO reacted with dissolved oxygen to form DMPO-OH. 2007

AB - When an aqueous solution of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was heated at 70°C for 30 min, formation of DMPO-OH was observed by ESR. This DMPO-OH radical formation was suppressed under an argon atmosphere. When water was replaced with ultra-pure water for ICP-MS experiments, DMPO-OH radical formation was also diminished. Under an argon atmosphere in ultra-pure water, the intensity of the DMPO-OH signal decreased to about 1/20 of that observed under aerobic conditions with regular purified water. The addition of hydroxyl radical scavengers such as mannitol did not affect the formation of DMPO-OH, but the signal turned faint in the presence of EDTA. We suggest that DMPO reacted with dissolved oxygen to form DMPO-OH. 2007

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DMPO-OH radical formation from 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in hot water

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