DMPO-OH radical formation from 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in hot water

Tomoko Shoji, Linxiang Li, Yoshihiro Abe, Masahiro Ogata, Yoshihisa Ishimoto, Ryoko Gonda, Tadahiko Mashino, Masataka Mochizuki, Michihisa Uemoto, Naoki Miyata

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Abstract

When an aqueous solution of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was heated at 70°C for 30 min, formation of DMPO-OH was observed by ESR. This DMPO-OH radical formation was suppressed under an argon atmosphere. When water was replaced with ultra-pure water for ICP-MS experiments, DMPO-OH radical formation was also diminished. Under an argon atmosphere in ultra-pure water, the intensity of the DMPO-OH signal decreased to about 1/20 of that observed under aerobic conditions with regular purified water. The addition of hydroxyl radical scavengers such as mannitol did not affect the formation of DMPO-OH, but the signal turned faint in the presence of EDTA. We suggest that DMPO reacted with dissolved oxygen to form DMPO-OH. 2007

Original languageEnglish
Pages (from-to)219-221
Number of pages3
JournalAnalytical Sciences
Volume23
Issue number2
DOIs
Publication statusPublished - 2007 Feb

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ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Shoji, T., Li, L., Abe, Y., Ogata, M., Ishimoto, Y., Gonda, R., Mashino, T., Mochizuki, M., Uemoto, M., & Miyata, N. (2007). DMPO-OH radical formation from 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in hot water. Analytical Sciences, 23(2), 219-221. https://doi.org/10.2116/analsci.23.219