DNA-barcode directed capture and electrochemical metabolic analysis of single mammalian cells on a microelectrode array

Erik S. Douglas, Sonny C. Hsiao, Hiroaki Onoe, Carolyn R. Bertozzi, Matthew B. Francis, Richard A. Mathies

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

A microdevice is developed for DNA-barcode directed capture of single cells on an array of pH-sensitive microelectrodes for metabolic analysis. Cells are modified with membrane-bound single-stranded DNA, and specific single-cell capture is directed by the complementary strand bound in the sensor area of the iridium oxide pH microelectrodes within a microfluidic channel. This bifunctional microelectrode array is demonstrated for the pH monitoring and differentiation of primary T cells and Jurkat T lymphoma cells. Single Jurkat cells exhibited an extracellular acidification rate of 11 milli-pH min -1, while primary T cells exhibited only 2 milli-pH min-1. This system can be used to capture non-adherent cells specifically and to discriminate between visually similar healthy and cancerous cells in a heterogeneous ensemble based on their altered metabolic properties.

Original languageEnglish
Pages (from-to)2010-2015
Number of pages6
JournalLab on a Chip
Volume9
Issue number14
DOIs
Publication statusPublished - 2009

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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