DNA-carrying latex particles for DNA diagnosis. 1. Separation of normal and point mutant DNAs according to the difference in hybridization efficiency

M. Hatakeyama, S. Iwato, Keiji Fujimoto, H. Handa, H. Kawaguchi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Point mutation of specific genome DNA causes cancer. A system for rapid and sensitive detection of specific genome DNA is required because it enables early detection and treatment of cancer. We developed a new system for DNA diagnosis using DNA-carrying latex particles and succeeded to collect point mutant specifically from the mixture with normal DNA of codon 12 of K-ras gene.

Original languageEnglish
Pages (from-to)161-169
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume10
Issue number3
DOIs
Publication statusPublished - 1998 Feb 15

Fingerprint

Latex
latex
Latexes
Microspheres
Particles (particulate matter)
DNA
deoxyribonucleic acid
genome
Genes
cancer
Genome
ras Genes
mutations
Early Detection of Cancer
Point Mutation
Codon
genes
causes
Neoplasms

Keywords

  • Affinity latex
  • DNA diagnosis
  • Hybridization
  • Microsphere
  • Point mutation

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

DNA-carrying latex particles for DNA diagnosis. 1. Separation of normal and point mutant DNAs according to the difference in hybridization efficiency. / Hatakeyama, M.; Iwato, S.; Fujimoto, Keiji; Handa, H.; Kawaguchi, H.

In: Colloids and Surfaces B: Biointerfaces, Vol. 10, No. 3, 15.02.1998, p. 161-169.

Research output: Contribution to journalArticle

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