DNA-carrying latex particles for DNA diagnosis 2. Distinction of normal and point mutant DNA using S1 nuclease

M. Hatakeyama, K. Nakamura, S. Iwato, H. Handa, K. Fujimoto, H. Kawaguchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In our previous paper, we reported a DNA diagnosis system in which fully complementary DNA was selectively hybridized with a DNA immobilized on latex particles but one-point mutant DNA was not. The system, however, included a few disadvantages. To overcome them, a single-strand DNA cleaving enzyme (S1 nuclease) was used in this study. S1 nuclease cleaved the unhybridized and loosely hybridized DNA's on the particles and enabled the determination of the fully complementary hybrid. This method saved us a troublesome labeling of the samples.

Original languageEnglish
Pages (from-to)171-178
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume10
Issue number3
DOIs
Publication statusPublished - 1998 Feb 15

Keywords

  • DNA diagnosis
  • Microsphere
  • Miss-matched DNA
  • Point-mutation
  • S1 nuclease

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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