Abstract
We have developed DNA-carrying affinity latex particles for distinction of point-mutational genes, but point-mutational DNA was not full length in the sample obtained from patients and had to be labeled. To overcome this disadvantage, we used an indirect labeled probe in this study. The indirect labeled probe was complementary with a 5' end region of 70mer DNA involved in the K-ras gene. The indirect labeled probe did not play a role of direct detection of point-mutational DNA, but the complex of 70mer DNA and an indirect labeled probe hybridized with single-stranded DNA immobilized on latex particles. This study suggests practical DNA diagnosis by using DNA-carrying affinity latex. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 445-451 |
| Number of pages | 7 |
| Journal | Colloids and Surfaces A: Physicochemical and Engineering Aspects |
| Volume | 153 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - 1999 Aug 15 |
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Keywords
- Affinity latex
- DNA diagnosis
- Indirect labeled probe
- Point mutation
ASJC Scopus subject areas
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
Cite this
DNA-carrying latex particles for DNA diagnosis 3. Detection of point mutant DNA using an indirect labeled probe. / Hatakeyama, M.; Iwato, S.; Hanashita, H.; Nakamura, K.; Fujimoto, Keiji; Kawaguchi, H.
In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 153, No. 1-3, 15.08.1999, p. 445-451.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA-carrying latex particles for DNA diagnosis 3. Detection of point mutant DNA using an indirect labeled probe
AU - Hatakeyama, M.
AU - Iwato, S.
AU - Hanashita, H.
AU - Nakamura, K.
AU - Fujimoto, Keiji
AU - Kawaguchi, H.
PY - 1999/8/15
Y1 - 1999/8/15
N2 - We have developed DNA-carrying affinity latex particles for distinction of point-mutational genes, but point-mutational DNA was not full length in the sample obtained from patients and had to be labeled. To overcome this disadvantage, we used an indirect labeled probe in this study. The indirect labeled probe was complementary with a 5' end region of 70mer DNA involved in the K-ras gene. The indirect labeled probe did not play a role of direct detection of point-mutational DNA, but the complex of 70mer DNA and an indirect labeled probe hybridized with single-stranded DNA immobilized on latex particles. This study suggests practical DNA diagnosis by using DNA-carrying affinity latex. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.
AB - We have developed DNA-carrying affinity latex particles for distinction of point-mutational genes, but point-mutational DNA was not full length in the sample obtained from patients and had to be labeled. To overcome this disadvantage, we used an indirect labeled probe in this study. The indirect labeled probe was complementary with a 5' end region of 70mer DNA involved in the K-ras gene. The indirect labeled probe did not play a role of direct detection of point-mutational DNA, but the complex of 70mer DNA and an indirect labeled probe hybridized with single-stranded DNA immobilized on latex particles. This study suggests practical DNA diagnosis by using DNA-carrying affinity latex. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.
KW - Affinity latex
KW - DNA diagnosis
KW - Indirect labeled probe
KW - Point mutation
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UR - http://www.scopus.com/inward/citedby.url?scp=0032813727&partnerID=8YFLogxK
U2 - 10.1016/S0927-7757(98)00466-X
DO - 10.1016/S0927-7757(98)00466-X
M3 - Article
VL - 153
SP - 445
EP - 451
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
SN - 0927-7757
IS - 1-3
ER -