DNA-carrying latex particles for DNA diagnosis 3. Detection of point mutant DNA using an indirect labeled probe

M. Hatakeyama; S. Iwato; H. Hanashita; K. Nakamura; K. Fujimoto; H. Kawaguchi

doi:10.1016/S0927-7757(98)00466-X

DNA-carrying latex particles for DNA diagnosis 3. Detection of point mutant DNA using an indirect labeled probe

M. Hatakeyama, S. Iwato, H. Hanashita, K. Nakamura, K. Fujimoto, H. Kawaguchi

Department of Applied Chemistry

Research output: Contribution to journal › Conference article

13 Citations (Scopus)

Abstract

We have developed DNA-carrying affinity latex particles for distinction of point-mutational genes, but point-mutational DNA was not full length in the sample obtained from patients and had to be labeled. To overcome this disadvantage, we used an indirect labeled probe in this study. The indirect labeled probe was complementary with a 5' end region of 70mer DNA involved in the K-ras gene. The indirect labeled probe did not play a role of direct detection of point-mutational DNA, but the complex of 70mer DNA and an indirect labeled probe hybridized with single-stranded DNA immobilized on latex particles. This study suggests practical DNA diagnosis by using DNA-carrying affinity latex. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	445-451
Number of pages	7
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	153
Issue number	1-3
DOIs	https://doi.org/10.1016/S0927-7757(98)00466-X
Publication status	Published - 1999 Aug 15
Event	Proceedings of the 1997 7th Iketani Conference - International Symposium on Advanced Technology of Fine Particles - Yokohama, Jpn Duration: 1997 Oct 12 → 1997 Oct 16

Keywords

Affinity latex
DNA diagnosis
Indirect labeled probe
Point mutation

ASJC Scopus subject areas

Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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Hatakeyama, M., Iwato, S., Hanashita, H., Nakamura, K., Fujimoto, K., & Kawaguchi, H. (1999). DNA-carrying latex particles for DNA diagnosis 3. Detection of point mutant DNA using an indirect labeled probe. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 153(1-3), 445-451. https://doi.org/10.1016/S0927-7757(98)00466-X

DNA-carrying latex particles for DNA diagnosis 3. Detection of point mutant DNA using an indirect labeled probe. / Hatakeyama, M.; Iwato, S.; Hanashita, H.; Nakamura, K.; Fujimoto, K.; Kawaguchi, H.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 153, No. 1-3, 15.08.1999, p. 445-451.

Research output: Contribution to journal › Conference article

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