DNA-carrying latex particles for DNA diagnosis 3. Detection of point mutant DNA using an indirect labeled probe

M. Hatakeyama, S. Iwato, H. Hanashita, K. Nakamura, Keiji Fujimoto, H. Kawaguchi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have developed DNA-carrying affinity latex particles for distinction of point-mutational genes, but point-mutational DNA was not full length in the sample obtained from patients and had to be labeled. To overcome this disadvantage, we used an indirect labeled probe in this study. The indirect labeled probe was complementary with a 5' end region of 70mer DNA involved in the K-ras gene. The indirect labeled probe did not play a role of direct detection of point-mutational DNA, but the complex of 70mer DNA and an indirect labeled probe hybridized with single-stranded DNA immobilized on latex particles. This study suggests practical DNA diagnosis by using DNA-carrying affinity latex. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)445-451
Number of pages7
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume153
Issue number1-3
DOIs
Publication statusPublished - 1999 Aug 15

Fingerprint

Latex
latex
Latexes
Particles (particulate matter)
DNA
deoxyribonucleic acid
probes
genes
Genes
affinity
Single-Stranded DNA

Keywords

  • Affinity latex
  • DNA diagnosis
  • Indirect labeled probe
  • Point mutation

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

DNA-carrying latex particles for DNA diagnosis 3. Detection of point mutant DNA using an indirect labeled probe. / Hatakeyama, M.; Iwato, S.; Hanashita, H.; Nakamura, K.; Fujimoto, Keiji; Kawaguchi, H.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 153, No. 1-3, 15.08.1999, p. 445-451.

Research output: Contribution to journalArticle

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