DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells

Nobuhide Doi, Natsuko Yamakawa, Hideaki Matsumoto, Yasutsugu Yamamoto, Tetsuya Nagano, Nobutaka Matsumura, Kenichi Horisawa, Hiroshi Yanagawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The G protein-coupled receptors (GPCRs), which form the largest group of transmembrane proteins involved in signal transduction, are major targets of currently available drugs. Thus, the search for cognate and surrogate peptide ligands for GPCRs is of both basic and therapeutic interest. Here we describe the application of an in vitro DNA display technology to screening libraries of peptide ligands for full-length GPCRs expressed on whole cells. We used human angiotensin II (Ang II) type-1 receptor (hAT1R) as a model GPCR. Under improved selection conditions using hAT1R-expressing Chinese hamster ovary (CHO)-K1 cells as bait, we confirmed that Ang II gene could be enriched more than 10,000-fold after four rounds of selection. Further, we successfully selected diverse Ang II-like peptides from randomized peptide libraries. The results provide more precise information on the sequence-function relationships of hAT1R ligands than can be obtained by conventional alanine-scanning mutagenesis. Completely in vitro DNA display can overcome the limitations of current display technologies and is expected to prove widely useful for screening diverse libraries of mutant peptide and protein ligands for receptors that can be expressed functionally on the surface of CHO-K1 cells.

Original languageEnglish
Article numbere30084
JournalPLoS One
Volume7
Issue number1
DOIs
Publication statusPublished - 2012 Jan 10

Fingerprint

Chinese hamsters
G-Protein-Coupled Receptors
Cricetulus
Peptide Library
angiotensin II
Ovary
Display devices
peptides
peptide libraries
Ligands
Peptides
DNA
Angiotensin II
Screening
cells
screening
Technology
Signal transduction
Mutagenesis
Angiotensin Type 1 Receptor

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Doi, N., Yamakawa, N., Matsumoto, H., Yamamoto, Y., Nagano, T., Matsumura, N., ... Yanagawa, H. (2012). DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells. PLoS One, 7(1), [e30084]. https://doi.org/10.1371/journal.pone.0030084

DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells. / Doi, Nobuhide; Yamakawa, Natsuko; Matsumoto, Hideaki; Yamamoto, Yasutsugu; Nagano, Tetsuya; Matsumura, Nobutaka; Horisawa, Kenichi; Yanagawa, Hiroshi.

In: PLoS One, Vol. 7, No. 1, e30084, 10.01.2012.

Research output: Contribution to journalArticle

Doi, N, Yamakawa, N, Matsumoto, H, Yamamoto, Y, Nagano, T, Matsumura, N, Horisawa, K & Yanagawa, H 2012, 'DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells', PLoS One, vol. 7, no. 1, e30084. https://doi.org/10.1371/journal.pone.0030084
Doi, Nobuhide ; Yamakawa, Natsuko ; Matsumoto, Hideaki ; Yamamoto, Yasutsugu ; Nagano, Tetsuya ; Matsumura, Nobutaka ; Horisawa, Kenichi ; Yanagawa, Hiroshi. / DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells. In: PLoS One. 2012 ; Vol. 7, No. 1.
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