DNA Hybridization Assay Using Gold Nanoparticles and Electrophoresis Separation Provides 1 pM Sensitivity

Keiko Esashika, Toshiharu Saiki

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    The efficiency of gold nanoparticle (AuNP) dimerization mediated by hybridization between two probe DNA molecules and a complementary target DNA molecule was maximized by examining several possible hybridization combinations. The uniformity of the size of the AuNPs, the use of surface modification appropriate for high hybridization efficiency, together with efficient blocking of nonspecific binding, all contributed to achieving a 1 pM detection limit following conventional gel electrophoresis separation of the DNA-modified AuNP multimers. This practical homogeneous DNA hybridization assay methodology will provide a rapid, cost-effective, and field-portable tool for clinical diagnosis.

    Original languageEnglish
    Pages (from-to)182-189
    Number of pages8
    JournalBioconjugate Chemistry
    Volume29
    Issue number1
    DOIs
    Publication statusPublished - 2018 Jan 17

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Biomedical Engineering
    • Pharmacology
    • Pharmaceutical Science
    • Organic Chemistry

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