DNA Hybridization Assay Using Gold Nanoparticles and Electrophoresis Separation Provides 1 pM Sensitivity

Keiko Esashika, Toshiharu Saiki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The efficiency of gold nanoparticle (AuNP) dimerization mediated by hybridization between two probe DNA molecules and a complementary target DNA molecule was maximized by examining several possible hybridization combinations. The uniformity of the size of the AuNPs, the use of surface modification appropriate for high hybridization efficiency, together with efficient blocking of nonspecific binding, all contributed to achieving a 1 pM detection limit following conventional gel electrophoresis separation of the DNA-modified AuNP multimers. This practical homogeneous DNA hybridization assay methodology will provide a rapid, cost-effective, and field-portable tool for clinical diagnosis.

Original languageEnglish
Pages (from-to)182-189
Number of pages8
JournalBioconjugate Chemistry
Volume29
Issue number1
DOIs
Publication statusPublished - 2018 Jan 17

Fingerprint

Electrophoresis
Gold
Nanoparticles
Assays
DNA
DNA Probes
Dimerization
Limit of Detection
Molecules
Complementary DNA
Gels
Costs and Cost Analysis
Surface treatment
Costs

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

DNA Hybridization Assay Using Gold Nanoparticles and Electrophoresis Separation Provides 1 pM Sensitivity. / Esashika, Keiko; Saiki, Toshiharu.

In: Bioconjugate Chemistry, Vol. 29, No. 1, 17.01.2018, p. 182-189.

Research output: Contribution to journalArticle

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