DNA hypermethylation at the D17S5 locus in non-small cell lung cancers

Its association with smoking history

Keisuke Eguchi, Yae Kanai, Koichi Kobayashi, Setsuo Hirohashi

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The aim of this study was to examine the association between DNA hypermethylation and clinicopathological features of non-small cell lung cancers (NSCLCs). The DNA methylation status at the D17S5 loci, at which a candidate tumor suppressor gene, HIC-1 (hypermethylated in cancer), was identified, of 51 paired tumor and nontumorous lung tissue specimens from NSCLC patients were examined by Southern blot analysis, using a methylation- sensitive restriction enzyme. DNA hypermethylation at this locus was found in 17 (33%) tumors and 16 (31%) nontumorous lung tissues. DNA in hypermethylation at this locus occurred more frequently in poorly than in well-differentiated tumors, especially in adenocarcinomas, and correlated significantly with the differentiation grade (P = 0.01). DNA hypermethylation at the D17S5 locus correlated significantly with the loss of heterozygosity at this locus in tumors (P = 0.01). The incidence of DNA hypermethylation was significantly higher in smokers than those who had never smoked in both tumors and nontumorous lung tissues (P = 0.03 and P = 0.01, respectively). These results suggest that DNA hypermethylation at the D17S5 locus may play a role in the development of NSCLCs in cigarette smokers.

Original languageEnglish
Pages (from-to)4913-4915
Number of pages3
JournalCancer Research
Volume57
Issue number21
Publication statusPublished - 1997 Nov 1
Externally publishedYes

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Non-Small Cell Lung Carcinoma
Smoking
History
DNA
Neoplasms
Lung
Loss of Heterozygosity
DNA Methylation
Southern Blotting
Tumor Suppressor Genes
Tobacco Products
Methylation
Adenocarcinoma
Incidence
Enzymes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

DNA hypermethylation at the D17S5 locus in non-small cell lung cancers : Its association with smoking history. / Eguchi, Keisuke; Kanai, Yae; Kobayashi, Koichi; Hirohashi, Setsuo.

In: Cancer Research, Vol. 57, No. 21, 01.11.1997, p. 4913-4915.

Research output: Contribution to journalArticle

Eguchi, Keisuke ; Kanai, Yae ; Kobayashi, Koichi ; Hirohashi, Setsuo. / DNA hypermethylation at the D17S5 locus in non-small cell lung cancers : Its association with smoking history. In: Cancer Research. 1997 ; Vol. 57, No. 21. pp. 4913-4915.
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