DNA methyltransferase 3B expression is associated with poor outcome of stage I testicular seminoma

Eri Arai, Tohru Nakagawa, Saori Wakai-Ushijima, Hiroyuki Fujimoto, Yae Kanai

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Aims: To examine in testicular seminomas the expression of DNA methyltransferase 3B (DNMT3B), which is known to be associated with early embryonic development and carcinogenesis, and to obtain a predictive marker for relapse of stage I seminomas. Methods and results: Immunohistochemical examination of DNMT3B was performed in 88 cases of seminoma, 35 (39.8%) of which showed widely scattered nuclear immunoreactivity for DNMT3B, and 53 (60.2%) of which were completely negative. The incidence of focal DNMT3B expression was higher in stage III seminomas (5/5, 100%) than in stage I (25/70, 35.7%) or stage II (5/13, 38.5%) seminomas (P=0.011). In stage I seminomas there were no significant correlations between DNMT3B expression and tumour size, invasion of the rete testis, or lymphatic or vascular involvement. Six of 25 cases (24%) showing DNMT3B expression relapsed, whereas only 3/45 cases (6.7%) lacking such expression did so (P=0.037). Patients with seminomas showing DNMT3B expression had a significantly lower relapse-free survival rate than patients whose tumours lacked this feature (P=0.0464). Conclusions: Patients with seminomas showing focal DNMT3B expression are at increased risk of relapse, and should be followed up carefully.

Original languageEnglish
Pages (from-to)E12-E18
JournalHistopathology
Volume60
Issue number6 B
DOIs
Publication statusPublished - 2012 May 1
Externally publishedYes

Keywords

  • DNA methyltransferase 3B
  • Prognostication
  • Seminoma
  • Testicular germ cell tumour
  • Tumour relapse

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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